PXD019488-2

PXD019488 is an original dataset announced via ProteomeXchange.

Title	Identification of TbUnc119-interacting proteins by proximity-based biotinylation
Description	Both intraflagellar transport (IFT) and lipidated intraflagellar transport (LIFT) pathways are essential for cilia/flagella biogenesis, motility and sensory functions. In the LIFT pathway, lipidated cargoes are transported into the cilia through the coordinated actions of cargo carrier proteins such as Unc119 or PDE6δ, as well as small GTPases Arl13b and Arl3 in the cilium. Our previous studies revealed a single Arl13b ortholog in the evolutionarily divergent Trypanosoma brucei. TbArl13 catalyses two TbArl3 homologs, TbArl3A and TbArl3C, suggesting the presence of a conserved LIFT pathway in these protozoan parasites. Only a single homolog to the cargo carrier protein Unc119 was identified in T. brucei genome, but its function in lipidated protein transport has not been characterized. In this study, we exploited the proximity-based biotinylation approach to identify binding partners of TbUnc119 using an improved version of biotin ligase BioID2. The BioID2 tag was fused to either the N-terminus (3HA-BioID2-TbUnc119) or the C-terminus (TbUnc119-BioID2-HA) of TbUnc119 and expressed using a cumate-inducible expression system in the procyclic cells of T. brucei. We performed LC-MS/MS on the affinity-purified biotinylated samples followed by evaluation of emPAI score.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:43.478.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maneesha Pandey
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	methylthiolated residue; monohydroxylated residue; acetylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-01 03:10:51	ID requested
1	2020-06-24 23:04:55	announced
⏵ 2	2024-10-22 05:07:43	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Trypanosoma brucei,Flagellum, LC-MS/MS

Cynthia Y. He
contact affiliation	Department of Biological Sciences, Molecular and cellular parasitology lab, National University of Singapore,Singpapore
contact email	dbshyc@nus.edu.sg
lab head
Maneesha Pandey
contact affiliation	National University of Singapore
contact email	maneesha.pandey@u.nus.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019488
     1. Label: PRIDE project
     2. Name: Identification of TbUnc119-interacting proteins by proximity-based biotinylation