PXD019454-1

PXD019454 is an original dataset announced via ProteomeXchange.

Title	Plasmodium berghei LAPs form an extended protein complex that facilitates crystalloid targeting and biogenesis
Description	Passage of malaria parasites through mosquitoes involves multiple developmental transitions, from gametocytes that are ingested with the blood meal, through to sporozoites that are transmitted by insect bite to the host. During the transformation from gametocyte to oocyst, the parasite forms a unique transient organelle named the crystalloid, which is involved in sporozoite formation. In Plasmodium berghei, a complex of six LCCL domain-containing proteins (LAPs) reside in the crystalloid and are required for its biogenesis. However, little else is known about the molecular mechanisms that underlie the crystalloid's role in sporogony. In this study, we have used transgenic parasites stably expressing LAP3 fused to GFP, combined with GFP affinity pulldown and high accuracy mass spectrometry, to identify an extended LAP interactome of some fifty proteins. We show that many of these are targeted to the crystalloid, including members of two protein families with CPW-WPC and pleckstrin homology-like domains, respectively. Our findings indicate that the LAPs are part of an intricate protein complex, the formation of which facilitates both crystalloid targeting and biogenesis.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_19:15:28.911.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vikram Sharma
SpeciesList	scientific name: Plasmodium berghei; NCBI TaxID: 5821;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-28 07:17:22	ID requested
⏵ 1	2021-09-08 19:15:29	announced
2	2024-10-22 05:27:37	announced	2024-10-22: Updated project metadata.

Tremp AZ, Saeed S, Sharma V, Lasonder E, Dessens JT, Plasmodium berghei LAPs form an extended protein complex that facilitates crystalloid targeting and biogenesis. J Proteomics, 227():103925(2020) [pubmed]

submitter keyword: Plasmodium, malaria, proteomics, crystalloid, LAPs

Vikram Sharma
contact affiliation	School of Biomedical Sciences, University of Plymouth, UK
contact email	vikram.sharma@plymouth.ac.uk
lab head
Vikram Sharma
contact affiliation	Plymouth University Peninsula Schools of Medicine and Dentistry
contact email	vikram.sharma@plymouth.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019454

PRIDE project URI

• PRIDE
  1. PXD019454
     1. Label: PRIDE project
     2. Name: Plasmodium berghei LAPs form an extended protein complex that facilitates crystalloid targeting and biogenesis