PXD019437-2
PXD019437 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Crosslinking/mass spectrometry analysis of Bacillus subtilis RNAP-Delta-HelD complexes |
| Description | Crosslinking mass spectrometry was applied to investigate protein contacts and dramatic structural rearrangements triggered by HelD binding to RNAP. Four complexes RNAPΔδΔHelD , RNAPΔδΔHelD-δ, RNAPΔδΔHelD-HelD and RNAPΔδΔHelD-δ-HelD were assembled using RNAP purified from B. subtilis ΔhelDΔropE strain (RNAPΔδΔHelD), and recombinant δ and HelD. The four complexes were crosslinked using a heterobifunctional, photoactivatable crosslinker sulfosuccinimidyl 4,4′‐azipentanoate (sulfo-SDA). Crosslinked protein complexes were analyzed by LC-MS/MS, and crosslinked residue pairs were identified in form of crosslinked peptides. |
| HostingRepository | jPOST |
| AnnounceDate | 2020-10-26 |
| AnnouncementXML | Submission_2021-02-04_16:59:30.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD019437 |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Zhuo Chen |
| SpeciesList | scientific name: cellular organisms; NCBI TaxID: 131567; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; unknown modification |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-05-28 01:28:56 | ID requested | |
| 1 | 2020-10-26 09:30:12 | announced | |
| ⏵ 2 | 2021-02-04 16:59:30 | announced | 2021-02-04: Updated FTP location. |
| 3 | 2021-12-21 07:23:17 | announced | 2021-12-22: Updated PubMed. |
| 4 | 2022-09-18 03:25:52 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Crosslinking/mass spectrometry, Bacillus RNAP, HelD |
Contact List
| Juri Rappsilber | |
|---|---|
| lab head | |
| Zhuo Chen | |
| contact affiliation | Technische Universität Berlin |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000858 |
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