PXD019426 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | High-density monitoring of tyrosine phosphorylation signaling targets in human tumors using internal standard triggered targeted quantitation |
Description | SureQuant pTyr, a targeted, tyrosine phosphorylation (pTyr) mass spectrometry method, was applied to profile the pTyr signatures of human colorectal tumor samples. Using internal standard guided aquisition with the SureQuant acquisition mode on the Oribtrap Exploris 480 mass spectrometer (Thermo Scientific), the real-time detection of trigger peptides during analysis initiates the sensitive and selective quantitation of endogenous pTyr targets. This framework allows for reliable quantification of several hundred commonly dysregulated pTyr targets with high quantitative accuracy and improves the robustness and usability of targeted aquisition. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:51:05.602.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lauren Stopfer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-27 17:12:09 | ID requested | |
1 | 2021-03-28 17:58:09 | announced | |
⏵ 2 | 2023-11-14 08:51:06 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: post-translational modification,Tyrosine phosphorylation, mass spectrometry, targeted, orbitrap, SureQuant |
Contact List
Forest White |
contact affiliation | Koch Institute of Integrative Cancer Research, Department of Biological Engineering, Massachusetts Institute of Technology |
contact email | fwhite@mit.edu |
lab head | |
Lauren Stopfer |
contact affiliation | MIT |
contact email | lstopfer@mit.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD019426 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019426
- Label: PRIDE project
- Name: High-density monitoring of tyrosine phosphorylation signaling targets in human tumors using internal standard triggered targeted quantitation