PXD019423-2

PXD019423 is an original dataset announced via ProteomeXchange.

Title	MS analysis of SARS-CoV2 proteins from patient samples
Description	We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. Our protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. https://biorxiv.org/cgi/content/short/2020.04.18.047878v1
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:51.864.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019423
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christian Ihling
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Met-loss; Deamidated; Met-loss+Acetyl; Oxidation; Acetyl; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-27 14:55:30	ID requested
1	2020-06-25 01:25:44	announced
⏵ 2	2024-10-22 05:07:57	announced	2024-10-22: Updated project metadata.

10.6019/PXD019423;

10.1021/acs.jproteome.0c00280;

Ihling C, T, ä, nzler D, Hagemann S, Kehlen A, H, ü, ttelmaier S, Arlt C, Sinz A, Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients. J Proteome Res, 19(11):4389-4392(2020) [pubmed]

ProteomeXchange project tag: Sars-cov-2, Covid-19, Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Sars-CoV-2, Covid-19

submitter keyword: COVID-19, Nucleoprotein, SARS-CoV-2, Mass spectrometry

Andrea Sinz
contact affiliation	Martin-Luther University Halle-Wittenberg Institute of Pharmacy Department of Pharmaceutical Chemistry & Bioanalytics Charles Tanford Protein Center
contact email	andrea.sinz@pharmazie.uni-halle.de
lab head
Christian Ihling
contact affiliation	MLU Halle, Inst. f. Pharmacy
contact email	christian.ihling@pharmazie.uni-halle.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019423

PRIDE project URI

• PRIDE
  1. PXD019423
     1. Label: PRIDE project
     2. Name: MS analysis of SARS-CoV2 proteins from patient samples