PXD019388-1

PXD019388 is an original dataset announced via ProteomeXchange.

Title	Global Epigenetic Analysis Reveals H3K27 Methylation as a Mediator of Double Strand Break Repair
Description	Ionizing Radiation is a ubiquitous cancer treatment modality which works in large part by inducing DNA Double Strand Breaks (DSBs). Recently, is has become apparent that chromatin surrounding DSBs is important for recognition of breaks, recruitment of repair factors, and timely DSB resolution. Here, we use tandem mass spectrometry proteomics to analyze global histone modification patterns in breast cancer cells after exposure to ionizing radiation. We find widespread and long-lasting changes to the epigenome. Specifically, we show that H3K27 trimethylation, a known regulator of cell fate and gene expression, increases after DNA damage. By microscopy, we show that these changes are rapid and occur proximate to sites of DNA damage. Lastly, using inhibitors of the H3K27 methyltransferase EZH2 we show that EZH2 is necessary for proper DNA damage recognition and survival of cells following IR exposure. Taken together, these results uncover suggest new, transcription-independent roles for epigenetic modifiers including EZH2 which could lead to novel chromatin biology.
HostingRepository	PRIDE
AnnounceDate	2026-07-16
AnnouncementXML	Submission_2026-07-16_11:54:05.037.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Donald Wolfgeher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	methylated residue; ubiquitination signature dipeptidyl lysine; phosphorylated residue; acetylated residue
Instrument	Q Exactive HF; TSQ Quantiva

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-26 01:11:54	ID requested
⏵ 1	2026-07-16 11:54:05	announced

Dataset with its publication pending

submitter keyword: Q-Exactive, H2K27Me3,MRM, Histone PTM, EpiProfile

Stephen J. Kron
contact affiliation	Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago IL, USA
contact email	skron@uchicago.edu
lab head
Donald Wolfgeher
contact affiliation	University of Chicago
contact email	donw@uchicago.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019388
     1. Label: PRIDE project
     2. Name: Global Epigenetic Analysis Reveals H3K27 Methylation as a Mediator of Double Strand Break Repair