PXD019362-1

PXD019362 is an original dataset announced via ProteomeXchange.

Title	Towards understandingTissue Mϕ heterogeneity and functions using quantitative proteomics
Description	Resident macrophages are important for maintaining tissue homeostasis and for defence against infections, but their precise functions in different tissues are not fully elucidated.We have used high resolution quantitative proteomics to investigate the functions of splenic red pulp macrophages and peritoneal cavity macrophages in the steady-state. The validation of several proteins at cellular expression levels by the flow cytometry analysis was consistently in agreement with the proteomics data. Peritoneal macrophages were shown to be enriched in a number of key enzymes and metabolic pathways normally associated with the liver, such as metabolism of fructose, detoxification, nitrogen homeostasis and the urea cycle. Supporting observations in proteomics, we find that PM are able to utilise glutamine and glutamate which are rich in peritoneum for urea generation. In comparison, splenic red pulp macrophages were enriched in proteins important for adaptive immunity such as antigen presenting MHC molecules, in addition to proteins required for erythrocyte homeostasis and iron turnover. We also show that these tissue macrophages may utilise carbon and nitrogen substrates for different metabolic fates to support distinct tissue-specific roles. This study provides a valuable resource for biologists interested in the functions of tissue macrophages.
HostingRepository	PRIDE
AnnounceDate	2020-06-24
AnnouncementXML	Submission_2020-06-24_06:25:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manjula Nagala
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-24 23:18:27	ID requested
⏵ 1	2020-06-24 06:25:25	announced
2	2024-10-22 05:07:45	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mouse, tissue, macrophages, LCMSMS

Professor Paul Crocker
contact affiliation	Professor of Glycoimmunology
contact email	p.r.crocker@dundee.ac.uk
lab head
Manjula Nagala
contact affiliation	Research Associate
contact email	mnagala@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019362

PRIDE project URI

• PRIDE
  1. PXD019362
     1. Label: PRIDE project
     2. Name: Towards understandingTissue Mϕ heterogeneity and functions using quantitative proteomics