PXD019333-2

PXD019333 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteome Analysis of Shewanella putrefaciens WS13 Mature Biofilm under Cold Stress
Description	Worldwide, Shewanella putrefaciens is the predominant seafood spoilage microorganism during cold storage. This bacterium can attach to biotic/abiotic surfaces to form biofilms which contribute to seafood quality degradation and shelf-life reduction. The mechanism of S. putrefaciens biofilm formation is not yet described. The variations of proteins measured by RM confirmed the importance of proteins during the formation of a mature biofilm. Comparative proteomic analysis showed that siderophore and iron chelate transport proteins were down-regulated during mature biofilm formation. The down-regulated aforementioned proteins are involved in promoting iron storage in response to a higher demand for metabolic energy, whereas, the upregulated proteins of the sulfur relay system, pyrimidine metabolism, and purine metabolism are related to bacterial adaptability. Synthesis of proteins related to cold stress was increased and proteins involved in aminoacyl-tRNA biosynthesis were up-regulated, whereas, proteins involved in aminopeptidase activity were down-regulated. Proteolysis to scavenge energy was reduced as proteins involved in pyrophosphatase activity were up-regulated. Also extracellular eDNA was found which may play an important role in maintaining the stability of mature S. putrefaciens biofilm structures under cold stress. This work provides a better understanding of the role of proteins in mature biofilms. In addition, the biofilm formation mechanism of a psychrotrophic spoilage bacterial species at low temperature is explored, which may contribute to generating biofilm controlling strategies during seafood preservation and processing.
HostingRepository	iProX
AnnounceDate	2020-05-21
AnnouncementXML	Submission_2023-03-01_18:39:30.735.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jun Yan
SpeciesList	scientific name: Shewanella putrefaciens; NCBI TaxID: 24;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-21 18:06:37	ID requested
1	2020-05-21 18:09:29	announced
⏵ 2	2023-03-01 18:39:31	announced	2023-03-02: Update publication information.

Yan J, Xie J, WS13 Mature Biofilm Under Cold Stress. Front Microbiol, 11():1225(2020) [pubmed]

submitter keyword: Shewanella putrefaciens, psychrotrophic bacteria, food spoilage, biofilm, cold stress, proteomics

Jing Xie
contact affiliation	Shanghai Ocean University
contact email	jxie@shou.edu.cn
lab head
Jun Yan
contact affiliation	Shanghai Ocean University
contact email	jyan@shou.edu.cn
dataset submitter

iProX dataset URI