PXD019323-1

PXD019323 is an original dataset announced via ProteomeXchange.

Title	Profiling the molecular states in the mouse ischemia and reperfusion cortex reveals a core regulation module of metabolic reprogramming
Description	Molecular changes caused by metabolic reprogramming in ischemia and reperfusion cortex remain an unaddressed issue. Applying the quantitative proteomic and lactylproteomic analysis, we found that the molecular changes of ischemic brain tissues are quite different between ischemia and reperfusion stages. Conjoint analysis with a previous published transcriptome data further showed that the significant changes in protein levels and protein lactylations were mainly correlated with neurons in the reperfusion and ischemia stages, respectively. The significant upregulated lactylation of Aldoc, one of the glycolytic enzymes, at lysine 230 may contribute to the metabolic reprogramming by regulating glycolysis of astrocyte in ischemia phase, and which may further promote the lactate-derived lactylation of protein lysine residues. Our findings lay the foundation for future mechanistic studies of metabolic reprogramming from a novel perspective of lactylation, and illustrate that protein lactylations may play important roles in regulating the biological functions of neural cells after ischemic stroke.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:49:37.711.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiao-Yi Xiong
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-21 00:35:20	ID requested
⏵ 1	2024-10-17 02:49:38	announced

Xiong XY, Pan XR, Luo XX, Wang YF, Zhang XX, Yang SH, Zhong ZQ, Liu C, Chen Q, Wang PF, Chen XW, Yu SG, Yang QW, Astrocyte-derived lactate aggravates brain injury of ischemic stroke in mice by promoting the formation of protein lactylation. Theranostics, 14(11):4297-4317(2024) [pubmed]

10.7150/thno.96375;

submitter keyword: Aldolase C, Ischemia, Metabolic reprogramming, Lactylation, Reperfusion

Xiao-Yi Xiong
contact affiliation	Chengdu University of Traditional Chinese Medicine
contact email	xiongxy1989@cdutcm.edu.cn
lab head
Xiao-Yi Xiong
contact affiliation	Acupuncture and Tuina School/Third Teaching Hospital, Chengdu University of Traditional Chinese Medicine
contact email	xiongxy_89@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019323
     1. Label: PRIDE project
     2. Name: Profiling the molecular states in the mouse ischemia and reperfusion cortex reveals a core regulation module of metabolic reprogramming