PXD019300-1
PXD019300 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | SARS-CoV-2_PRM_SRM_targeted_acquisitions |
| Description | We present here a high-throughput targeted proteomics assay to detect SARS-CoV-2 proteins directly from clinical respiratory tract samples. The use of turbulent flow chromatography included four times multiplexed on-line sample cleanup and UPLC separation. MS/MS detection of three peptides from SARS-CoV-2 nucleoprotein and a 15N-labeled internal global standard was achieved within 2.5 min, enabling the analysis of more than 500 samples per day. The method was validated using 562 specimens previously analyzed by real-time RT-PCR and was able to detect over 83% of positive cases. No interference was found with samples from common respiratory viruses, including other coronaviruses (NL63, OC43, HKU1, and 229E). In addition to the SRM method targeted to 3 peptides, two PRM methods used for peptides selection are also described. The strategy here presented has high sample stability and lower cost compared to real-time RT-PCR and could be considered as an option to large population SARS-CoV-2 testing. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2020-05-20 |
| AnnouncementXML | Submission_2020-05-20_20:16:53.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Valdemir Carvalho |
| SpeciesList | scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049; |
| ModificationList | Label:15N(3); Label:15N(1); Label:15N(2) |
| Instrument | Q Exactive HF-X; TSQ Altis |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-05-19 20:28:41 | ID requested | |
| ⏵ 1 | 2020-05-20 20:16:54 | announced | |
| 2 | 2020-09-16 09:39:17 | announced | : Added quantitative validation experiments |
| 3 | 2020-12-07 13:37:10 | announced | : Added PubMed ID |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SARS-CoV-2, COVID-19, targeted proteomics |
Contact List
| Valdemir Carvalho | |
|---|---|
| contact affiliation | Fleury Group |
| contact email | melechco@gmail.com |
| lab head | |
| Valdemir Carvalho | |
| contact affiliation | Fleury Group |
| contact email | melechco@gmail.com |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
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