PXD019284-1

PXD019284 is an original dataset announced via ProteomeXchange.

Title	Subclinical effects of remote ischaemic conditioning in human kidney transplants revealed by quantitative proteomics
Description	Background: Remote ischaemic conditioning (RIC) is currently being explored as a non-invasive method to attenuate ischaemia/reperfusion injuries in organs. A randomised clinical study (CONTEXT) evaluated the effects of RIC compared to non-RIC controls in human kidney transplants. Methods: RIC was induced prior to kidney reperfusion by episodes of obstruction to arterial flow in the l eg opposite the transplant using a tourniquet (4 x 5min). Although RIC did not lead to clinical improvement of transplant outcomes, we explored whether RIC induced molecular changes through precision analysis of CONTEXT recipient plasma and kidney tissue samples by high-resolution tandem mass spectrometry (MS/MS). Results: We observed an accumulation of muscle derived proteins in kidney tissue proteomes and altered mitochondrial proteins, likely provoked by RIC, which was not reflected in plasma. In addition, MS/MS analysis demonstrated transient upregulation of several acute phase response proteins (SAA1, SAA2, CRP) in plasma, 1 and 5 days post-transplant. RIC showed a lower magnitude change when compared to non-RIC. However, RIC did not have a consistent effect on the magnitude of acute inflammation following clinical intervention. Conclusions: Together, our results indicate sub-clinical systemic and organ-localised effects of RIC.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_00:05:35.810.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Darragh O'Brien
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-18 21:54:25	ID requested
⏵ 1	2021-09-09 00:05:36	announced

Thorne AM, Huang H, O'Brien DP, Eijken M, Krogstrup NV, Norregaard R, M, ø, ller B, Ploeg RJ, Jespersen B, Kessler BM, Subclinical effects of remote ischaemic conditioning in human kidney transplants revealed by quantitative proteomics. Clin Proteomics, 17(1):39(2020) [pubmed]

submitter keyword: remote ischaemic conditioning, kidney, quantitative proteomics

Benedikt M Kessler
contact affiliation	Target Discovery Institute Nuffield Department of Medicine
contact email	benedikt.kessler@ndm.ox.ac.uk
lab head
Darragh O'Brien
contact affiliation	Target Discovery Institute, University of Oxford
contact email	darragh.obrien@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019284
     1. Label: PRIDE project
     2. Name: Subclinical effects of remote ischaemic conditioning in human kidney transplants revealed by quantitative proteomics