PXD019280-1
PXD019280 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Precise temporal regulation of post-transcriptional repressors is required for an orderly Drosophila maternal-to-zygotic transition |
| Description | In animal embryos the maternal-to-zygotic transition (MZT) hands developmental control from maternal to zygotic gene products. We show that the maternal proteome represents over half of the protein coding capacity of the Drosophila melanogaster genome and that 2% of this proteome is rapidly degraded during the MZT. Cleared proteins include the post-transcriptional repressors Cup, Trailer hitch (TRAL), Maternal expression at 31B (ME31B), and Smaug (SMG). While the ubiquitin-proteasome system is necessary for clearance of all four repressors, distinct E3 ligase complexes target them: the C-terminal to Lis1 Homology (CTLH) complex targets Cup, TRAL and ME31B for degradation early in the MZT; the Skp/Cullin/F-box-containing (SCF) complex targets SMG at the end of the MZT. Deleting the C-terminal 233 amino acids of SMG makes the protein immune to degradation. We show that artificially persistent SMG downregulates the zygotic re-expression of mRNAs whose maternal contribution is cleared by SMG. Thus, clearance of SMG permits an orderly MZT |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-06-23 |
| AnnouncementXML | Submission_2020-06-23_04:17:37.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Sichun Lin |
| SpeciesList | scientific name: Drosophila melanogaster; common name: fruit fly; NCBI TaxID: 7227; |
| ModificationList | Oxidation |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-05-18 18:02:58 | ID requested | |
| ⏵ 1 | 2020-06-23 04:17:38 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Smaug, RNA-binding protein, ubiquitin ligase, Skp/Cullin/F-box-containing (SCF) complex, C-terminal to Lis1 Homology (CTLH) complex |
Contact List
| Howard Lipshitz | |
|---|---|
| contact affiliation | University of Toronto, |
| contact email | howard.lipshitz@utoronto.ca |
| lab head | |
| Sichun Lin | |
| contact affiliation | University of Toronto, Leslie Dan Faculty of Pharm |
| contact email | sichun.lin@utoronto.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085442/ |
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