PXD019273-2

PXD019273 is an original dataset announced via ProteomeXchange.

Title	Posttranslational modification of the RHO of plants protein RACB by phosphorylation and cross-kingdom conserved ubiquitination
Description	Small RHO-type G-proteins act as signaling hubs and master regulators of polarity in eukaryotic cells. Their activity is tightly controlled, as defective RHO signaling leads to aberrant growth and developmental defects. Two major pathways regulate G-protein activity: canonical switching of the nucleotide bound state and posttranslational modification (PTM). PTMs can support or suppress RHO signaling, depending on each individual case. In plants, regulation of Rho of plants (ROPs) has been shown to act through nucleotide exchange and hydrolysis, as well as through lipid modification, but there is little data available on phosphorylation or ubiquitination of ROPs. Hence, we applied proteomic analyses to identify PTMs of the barley ROP RACB. Data show in vitro phosphorylation by barley ROP Binding Kinase 1 and in vivo ubiquitination of RACB.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:29:52.316.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Mergner
SpeciesList	scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	ubiquitinylated lysine; phosphorylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-18 17:04:29	ID requested
1	2021-11-02 04:46:25	announced
⏵ 2	2024-10-22 05:29:52	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: RHO-type G-protein, ubiquitination, phosphorylation, Barley

Bernhard Kuster
contact affiliation	TUM
contact email	kuster@tum.de
lab head
Julia Mergner
contact affiliation	Chair of Proteomics and Bioanalytics, TU Munich
contact email	julia.mergner@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019273
     1. Label: PRIDE project
     2. Name: Posttranslational modification of the RHO of plants protein RACB by phosphorylation and cross-kingdom conserved ubiquitination