PXD019239-1

PXD019239 is an original dataset announced via ProteomeXchange.

Title	STING mediates lupus via the activation of conventional dendritic cell maturation and plasmacytoid dendritic cell differentiation
Description	BMDCs from WT, Fcgr2b-/- and Stinggt/gt were cultured, then stimulated with DMXAA for 3 and 6 hr. Cells were collected and lysed with 1 % IGEPAL CA-630, 0.5% TritonX-100, 150 mM NaCl, 50 mM Tris pH 7.4, 5% glycerol, 100 mM beta-Glycerophosphate, 2 mM Na3VO4 and 1X proteases inhibitor cocktail (Roche). First, antibody were mixed with the magnetic beads by adding 10 µg of STING antibody with 400 ug of SureBeads™ Protein A magnetic beads (Biorad, California, USA) and incubated for 1 hour at room temperature. Then, Protein lysates were added and incubated with antibody-conjugated beads for overnight at 4oC. After incubation, the beads were washed 3 times with wash buffer (150 mM NaCl and 50 mM Tris-HCL pH 7.4). Samples were eluted by adding 5X laemmli buffer and, boiled 950C for 10 minutes. The eluted protein samples were separated by 10 % SDS-PAGE gel. The STING interacting proteins from co-IP were analyzed by in-gel digestion followed by LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2020-09-14
AnnouncementXML	Submission_2020-09-13_22:27:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Poorichaya Somparn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-17 18:07:01	ID requested
⏵ 1	2020-09-13 22:27:59	announced
2	2024-10-22 05:12:18	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: Human Proteome Project

submitter keyword: Lupus, STING, Interferon, Fcgr2b, LYN, Dendritic cell

Poorichaya Somparn
contact affiliation	Center of Excellence in Systems Biology, Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
contact email	Poorichaya.s@chula.ac.th
lab head
Poorichaya Somparn
contact affiliation	Chulalongkorn university
contact email	poorichaya.s@chula.ac.th
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD019239

PRIDE project URI

• PRIDE
  1. PXD019239
     1. Label: PRIDE project
     2. Name: STING mediates lupus via the activation of conventional dendritic cell maturation and plasmacytoid dendritic cell differentiation