PXD019204 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An azidoribose probe to track ketoamine adducts in histone ribose glycation (293T nuclei - LFQ) |
Description | Reactive cellular metabolites can modify macromolecules and form adducts known as non-enzymatic covalent modifications (NECMs). Dissecting the mechanisms, regulation and consequences of NECMs, such as glycation, has been challenging due to the complex and often ambiguous nature of the adducts formed. Directly tracking the formation of modifications on key targets to uncover their underlying physiological importance requires specific chemical tools. Here we present the novel chemoenzymatic syntheses of an active azido-modified ribose analog, 5-azidoribose (5-AR), as well as an inactive control derivative, 1-azidoribose (1-AR) and their application towards understanding protein ribose-glycation in vitro and in cellulo. With these new probes we found that, similar to MGO-glycation, ribose glycation specifically accumulates on histones. In addition to fluorescent labeling, we demonstrate the utility of the probe in enriching modified targets, which were identified by label-free quantitative proteomics and highresolution MS/MS workflows. Finally, we establish that the known oncoprotein and hexose deglycase, fructosamine 3-kinase (FN3K), recognizes and facilitates the removal of 5-AR glycation adducts in live cells, supporting the dynamic regulation of ribose glycation as well as validating the probe as a new chemical tool to monitor FN3K activity. Altogether, we demonstrate this probe’s utilities to uncover ribose-glycation and deglycation events as well as tracking FN3K activity towards establishing its potential as a new cancer vulnerability. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-15 |
AnnouncementXML | Submission_2020-05-15_03:02:10.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Igor Maksimovic |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-14 07:19:31 | ID requested | |
⏵ 1 | 2020-05-15 03:02:11 | announced | |
Publication List
Maksimovic I, Zheng Q, Trujillo MN, Galligan JJ, David Y, An Azidoribose Probe to Track Ketoamine Adducts in Histone Ribose Glycation. J Am Chem Soc, 142(22):9999-10007(2020) [pubmed] |
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
submitter keyword: Histones, ribose, glycation, click chemistry, epigenetics, proteomics |
Contact List
Yael David |
contact affiliation | Tri-Institutional Program, New York, NY, United States; Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, United States; Department of Pharmacology, Weill Cornell Medical College, New York, NY, United States; Department of Physiology, Biophysics and Systems Biology, Weill Cornell Medical College, New York, NY, United States |
contact email | davidshy@mskcc.org |
lab head | |
Igor Maksimovic |
contact affiliation | Tri-Institutional PhD Program in Chemical Biology |
contact email | igm2002@med.cornell.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD019204 |
PRIDE project URI |
Repository Record List
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- PRIDE
- PXD019204
- Label: PRIDE project
- Name: An azidoribose probe to track ketoamine adducts in histone ribose glycation (293T nuclei - LFQ)