PXD019151 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Secretome characterization of nucleus pulposus (NP) cells subjected to ER stress |
Description | Objective: Induction of ER stress has been shown to promote NP cell apoptosis and disc degeneration. However, little is known about its regulation by hypoxia and its contribution to extracellular matrix homeostasis. Methods: NP cells were culture under hypoxia (1% O2) and normoxia (21% O2) to assess ER stress status. Tunicamycin and thapsigargin were used to induce canonical ER stress pathways and effects on cell secretome were assessed by proteomic analysis using mass spectrometry. The relevance of these findings to aging and degeneration was investigated by analyzing microarray data of NP tissues from aged mice (GSE134955) and degenerated human discs (GSE70362). Results: NP cells exhibited lower ER stress levels under hypoxia. ER stress inducers tunicamycin and thapsigargin promoted a canonical unfolded protein response. UPR further induced HIF-1 activity under hypoxia. NP cell secretome under ER stress showed an increased secretory pathway with a concomitant decrease in collagens, HAPLN1, and cell adhesion related proteins. Similar to our cell culture studies, NP tissues from aged mice and degenerated human discs presented higher levels of UPR markers and decreased levels of matrix components. |
HostingRepository | PRIDE |
AnnounceDate | 2020-12-03 |
AnnouncementXML | Submission_2020-12-03_02:43:32.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sandra Anjo |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | S-carboxamidoethyl-L-cysteine |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-12 22:35:05 | ID requested | |
⏵ 1 | 2020-12-03 02:43:32 | announced | |
2 | 2020-12-13 23:15:23 | announced | 2020-12-14: Updated publication reference for PubMed record(s): 33307037. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Nucleus pulposus, ER stress, secretome, hypoxia, collagens |
Contact List
Bruno Manadas |
contact affiliation | Center for Neuroscience and Cell Biology – University of Coimbra |
contact email | bmanadas@gmail.com |
lab head | |
Sandra Anjo |
contact affiliation | Center for Neuroscience and Cell Biology – University of Coimbra |
contact email | sandra.isabel.anjo@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019151
- Label: PRIDE project
- Name: Secretome characterization of nucleus pulposus (NP) cells subjected to ER stress