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PXD019132-1

PXD019132 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA carotenoid-deficient mutant of the plant-associated microbe Pantoea sp. YR343 displays an altered membrane proteome
DescriptionMembrane organization plays an important role in signaling, transport, and defense. In eukaryotes, the stability, organization, and function of membrane proteins are influenced by certain lipids and sterols, such as cholesterol. Bacteria lack cholesterol, but carotenoids and hopanoids are predicted to play a similar role in modulating membrane properties. We have previously shown that the loss of carotenoids in the plant-associated bacteria Pantoea sp. YR343 results in changes to membrane biophysical properties and leads to physiological changes, including increased sensitivity to reactive oxygen species, reduced indole-3-acetic acid secretion, reduced biofilm and pellicle formation, and reduced plant colonization. Here, using whole cell and membrane proteomics, we show that the deletion of carotenoid production in Pantoea sp. YR343 results in altered membrane protein distribution and abundance. Moreover, we observe significant differences in the protein composition of detergent-resistant membrane fractions from wildtype and mutant cells, consistent
HostingRepositoryMassIVE
AnnounceDate2020-09-22
AnnouncementXMLSubmission_2020-09-22_07:05:25.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPaul Abraham
SpeciesList scientific name: Pantoea sp. YR343; NCBI TaxID: 1144341;
ModificationListOxidation; Carbamidomethyl
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-12 12:29:30ID requested
12020-09-22 07:05:26announced
Publication List
no publication
Keyword List
submitter keyword: membrane proteome, plant-associated microbe
Contact List
Jennifer Morrell-Falvey
contact affiliationORNL
contact emailmorrelljl1@ornl.gov
lab head
Paul Abraham
contact affiliationORNL
contact emailpav@ornl.gov
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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