PXD019121-1

PXD019121 is an original dataset announced via ProteomeXchange.

Title	Cell wall proteome of Tuwongella immobilis
Description	Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and the ability to import macromolecules. These features raise intriguing questions about the composition of their cell envelopes. In this study, we have used microscopy, phylogenomics and proteomics to examine the composition and evolution of cell envelope proteins in Tuwongella immobilis and other members of the Planctomycetes. Cryo electron tomography data indicated a distance between the inner and outer membranes of 45 nm in T. immobilis. Consistent with the wide periplasmic space, our bioinformatics studies showed that the periplasmic segments of outer membrane proteins in type II secretion systems are extended in bacteria of the order Planctomycetales. Homologs of two highly abundant cysteine-rich cell wall proteins in T. immobilis were identified in all members of the Planctomycetales, whereas genes for peptidoglycan biosynthesis and cell elongation have been lost at least twice in this group of bacteria. The cell wall proteins contain multiple copies of the YTV motif, which is the only domain that is conserved and unique to the Planctomycetales. Earlier diverging taxa in the Plantomycetes phylum contain genes for peptidoglycan biosynthesis but no homologs to the YTV cell wall proteins. The major remodelling of the cell envelope in the ancestor of the Planctomycetales coincided with the emergence of budding and other unique cellular phenotypes. These results have implications for hypotheses about the process whereby complex cellular features evolve in bacteria.
HostingRepository	PRIDE
AnnounceDate	2020-08-10
AnnouncementXML	Submission_2020-08-10_04:23:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Seeger
SpeciesList	scientific name: Tuwongella immobilis; NCBI TaxID: 692036;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-12 05:59:08	ID requested
⏵ 1	2020-08-10 04:23:09	announced
2	2024-10-22 05:10:33	announced	2024-10-22: Updated project metadata.

Mahajan M, Seeger C, Yee B, Andersson SGE, Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum. Genome Biol Evol, 12(9):1528-1548(2020) [pubmed]

submitter keyword: Tuwongella immobilis, Planctomycetes, LC-MS/MS, cell wall

Siv Andersson
contact affiliation	Molecular Evolution, Department of Cell and Molecular Biology, Science for Life Laboratory, Biomedical Centre, Uppsala University, SE-751 24 Uppsala, Sweden
contact email	siv.andersson@icm.uu.se
lab head
Christian Seeger
contact affiliation	Department of Cell and Molecular Biology, Molecular Evolution, Uppsala University
contact email	christian.seeger@icm.uu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD019121

PRIDE project URI

• PRIDE
  1. PXD019121
     1. Label: PRIDE project
     2. Name: Cell wall proteome of Tuwongella immobilis