PXD019088 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Plasma exosomal proteomic pattern in Hemophagocyticlymphohistiocytosis of a twin boy |
Description | Background: Secondary HLH is closely related to EBV infection, protein changes in plasma exosomes may be related to the pathogenesis of HLH patient. In recent years quantitative proteomic has been used to clarify the relationship between basic medicine and clinical medicine. Methods: Exosomes were isolated from plasma samples of a HLH patient and his twin brother. Plasma samples were classified into three groups according to the clinical presentation, namely T1 (the acute HLH), T2 (during pharmacological treatments), Ctr (the healthy control). Herein, we used TMT-based LC-MS/MS platform to identified and characterized HLH signatures before and during pharmacological treatments. Results: Exosomes’ marker proteins- CD63, TSG101, HSP70 were identified by western blotting. Through GO analysis and KEGG analysis, we found that the signaling pathways and functions enriched by differentially expressed genes are very different. We focus on describing? the significantly up-regulated proteins-MSN, HSPA8, CD163, and the significantly down-regulated proteins- PLG, FGG, F2. The significant changes of these factors are related to the deterioration of the disease Conclusion: Our exploratory study shows that the clinical symptoms and pathophysiological changes of HLH driven by EBV might be reflected in patients’ plasma exosomes. Plasma exosome proteomics provides new highlightinsights for into exploring the diagnostic and therapeutic biomarkers of HLH. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-19 |
AnnouncementXML | Submission_2022-05-19_06:47:28.715.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yan Xie |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-11 07:51:28 | ID requested | |
⏵ 1 | 2022-05-19 06:47:30 | announced | |
Publication List
Xie Y, Yang L, Cao P, Li S, Zhang W, Dang W, Xin S, Jiang M, Xin Y, Li J, Long S, Wang Y, Zhang S, Yang Y, Lu J, Plasma Exosomal Proteomic Pattern of Epstein-Barr Virus-Associated Hemophagocytic Lymphohistiocytosis. Front Microbiol, 13():821311(2022) [pubmed] |
Keyword List
submitter keyword: Hemophagocyticlymphohistiocytosis; twins;exosomes;proteomic analysis |
Contact List
Yan Xie |
contact affiliation | 1Department of Hematology, Xiangya Hospital, Central South University, Changsha 410080, China 2NHC Key Laboratory of Carcinogenesis, Department of Microbiology, School of Basic Medical Science, Central South University, Changsha 410078, China |
contact email | 1148452190@qq.com |
lab head | |
Yan Xie |
contact affiliation | Central South University |
contact email | 1148452190@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019088
- Label: PRIDE project
- Name: Plasma exosomal proteomic pattern in Hemophagocyticlymphohistiocytosis of a twin boy