PXD019050-1

PXD019050 is an original dataset announced via ProteomeXchange.

Title	Identify proteins at Foxp3 bound cis-regulatory elements in situ
Description	We hypothesize that nuclear factors co-occupying the genetic elements with regulatory T (Treg) cell lineage–specifying factor Foxp3 play critical roles in transcriptional regulation of Treg immune suppression function, thus, offering a unique approach to investigate the factors and their mechanisms controlling Treg-mediated immune tolerance involved in self-tolerance and antitumor immunity. We seek to identify the proteins occupying Foxp3 targets in the resting state or after cells receiving stimulation. To this end, we projected the spatial information (PSI) of Foxp3, Histone H3, or Stat5 onto their adjacent proteins with peroxidase–catalyzed biotin-phenoxyl radicals and identify these biotinylated proteins with tandem mass tag (TMT)–based quantitative mass spectrometry (MS).
HostingRepository	PRIDE
AnnounceDate	2024-06-08
AnnouncementXML	Submission_2024-06-07_17:54:41.648.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vishwajeeth Pagala
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-07 22:45:56	ID requested
⏵ 1	2024-06-07 17:54:42	announced

10.1084/JEM.20232068;

submitter keyword: proximity ligation,Proteomics, Foxp3

Yongqiang Feng
contact affiliation	Assistant Member, Immunology Department St. Jude Children's Research Hospital Memphis, TN USA
contact email	yong.feng@stjude.org
lab head
Vishwajeeth Pagala
contact affiliation	St. Jude Children's Research Hospital
contact email	vishwajeeth.pagala@stjude.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD019050

PRIDE project URI

• PRIDE
  1. PXD019050
     1. Label: PRIDE project
     2. Name: Identify proteins at Foxp3 bound cis-regulatory elements in situ