PXD019043-2

PXD019043 is an original dataset announced via ProteomeXchange.

Title	Rab1b-AMPylation by Legionella DrrA is allosterically activated by Rab1
Description	Legionella pneumophila infect eukaryotic cells by forming a replicative organelle – the Legionella containing vacuole. During this process, the bacterial protein DrrA/SidM is secreted and manipulates the activity and posttranslational modification (PTM) states of the vesicular trafficking regulator Rab1. As a result, Rab1 is modified with an adenosine monophosphate (AMP) – a process referred to as AMPylation. Here, we used a chemical approach for stabilizing low affinity Rab:DrrA complexes in a site-specific manner to gain insights into the molecular basis of the interaction between the Rab protein and the AMPylation domain of DrrA. The X-ray crystal structure of the Rab:DrrA complex revealed a previously unrecognized non-conventional Rab binding site (NC-RBS). Biochemical characterizations demonstrated allosteric stimulation of DrrA’s AMPylation activity via Rab-binding to the NC-RBS. We propose that allosteric control of DrrA not only prevents random and potentially cytotoxic AMPylation in the host, thereby ensuring an efficient infection process by Legionella, but also represents an unprecedented AMPylation activation mechanism.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:17:15.321.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Stahl
SpeciesList	scientific name: Legionella pneumophila; NCBI TaxID: 446; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-07 22:28:55	ID requested
1	2021-01-04 03:53:00	announced
⏵ 2	2024-10-22 05:17:15	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: crosslink,Legionella, Rab, DrrA

Stephan Axel Sieber
contact affiliation	Center for Integrated Protein Science Munich (CIPSM) at the Department of Chemistry, Technische Universität München, Lichtenbergstraße 4, 85747 Garching b. München
contact email	stephan.sieber@tum.de
lab head
Matthias Stahl
contact affiliation	Karolinska Institutet
contact email	matthias.stahl@ki.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019043
     1. Label: PRIDE project
     2. Name: Rab1b-AMPylation by Legionella DrrA is allosterically activated by Rab1