PXD019042-1

PXD019042 is an original dataset announced via ProteomeXchange.

Title	Identification of immunoglobulin switch (S) region binding proteins by insertional ChIP (iChIP)
Description	We used insertional ChIP (iChIP) based approach to identify proteins that bind to mouse immunoglobulin switch (S) region. To perform iChIP, we inserted an 8X-repeat of the LexA-binding element (LexA-BE) downstream of the Sα region in CH12F3-2A cells, a mouse B-cell line. The DNA-binding domain and dimerization domain of the LexA protein fused with a FLAG tag and a nuclear localization signal (NLS) was expressed in WT (FNLDD-alone) or Sα-engineered (FNLDD-Sα-LexA) CH12F3-2A cells. The resultant cells were subjected to stable isotope labeling with amino acids in cell culture (SILAC), stimulated with CIT (CD40L, IL4, and TGFβ), immunoprecipitated with an anti-FLAG antibody, and subjected to LC-MS/MS analysis. The identities of the deposited data are as follows: F: WT (FNLDD-alone); D: Sα-engineered (FNLDD-Sα-LexA).
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:33:48.619.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fuminori Sugihara
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C; 6x(13)C labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-07 06:29:49	ID requested
⏵ 1	2024-10-07 13:33:49	announced

Husain A, Xu J, Fujii H, Nakata M, Kobayashi M, Wang JY, Rehwinkel J, Honjo T, Begum NA, SAMHD1-mediated dNTP degradation is required for efficient DNA repair during antibody class switch recombination. EMBO J, 39(15):e102931(2020) [pubmed]

10.15252/embj.2019102931;

submitter keyword: SAMHD1, SILAC, Recombination, CSR,Immunoglobulin locus, LC-MS/MS

Fuminori Sugihara
contact affiliation	Osaka Univ.
contact email	sugihara@biken.osaka-u.ac.jp
lab head
Fuminori Sugihara
contact affiliation	Osaka Univ.
contact email	sugihara@biken.osaka-u.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019042

PRIDE project URI

• PRIDE
  1. PXD019042
     1. Label: PRIDE project
     2. Name: Identification of immunoglobulin switch (S) region binding proteins by insertional ChIP (iChIP)