PXD019042 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of immunoglobulin switch (S) region binding proteins by insertional ChIP (iChIP) |
Description | We used insertional ChIP (iChIP) based approach to identify proteins that bind to mouse immunoglobulin switch (S) region. To perform iChIP, we inserted an 8X-repeat of the LexA-binding element (LexA-BE) downstream of the Sα region in CH12F3-2A cells, a mouse B-cell line. The DNA-binding domain and dimerization domain of the LexA protein fused with a FLAG tag and a nuclear localization signal (NLS) was expressed in WT (FNLDD-alone) or Sα-engineered (FNLDD-Sα-LexA) CH12F3-2A cells. The resultant cells were subjected to stable isotope labeling with amino acids in cell culture (SILAC), stimulated with CIT (CD40L, IL4, and TGFβ), immunoprecipitated with an anti-FLAG antibody, and subjected to LC-MS/MS analysis. The identities of the deposited data are as follows: F: WT (FNLDD-alone); D: Sα-engineered (FNLDD-Sα-LexA). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_13:33:48.619.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fuminori Sugihara |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | 6x(13)C; 6x(13)C labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-07 06:29:49 | ID requested | |
⏵ 1 | 2024-10-07 13:33:49 | announced | |
Publication List
Husain A, Xu J, Fujii H, Nakata M, Kobayashi M, Wang JY, Rehwinkel J, Honjo T, Begum NA, SAMHD1-mediated dNTP degradation is required for efficient DNA repair during antibody class switch recombination. EMBO J, 39(15):e102931(2020) [pubmed] |
10.15252/embj.2019102931; |
Keyword List
submitter keyword: SAMHD1, SILAC, Recombination, CSR,Immunoglobulin locus, LC-MS/MS |
Contact List
Fuminori Sugihara |
contact affiliation | Osaka Univ. |
contact email | sugihara@biken.osaka-u.ac.jp |
lab head | |
Fuminori Sugihara |
contact affiliation | Osaka Univ. |
contact email | sugihara@biken.osaka-u.ac.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019042 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019042
- Label: PRIDE project
- Name: Identification of immunoglobulin switch (S) region binding proteins by insertional ChIP (iChIP)