PXD018986-1

PXD018986 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The effect of pathogen inactivation on cryoprecipitate: A functional and quantitative evaluation
Description	As a pooled donor blood product, cryoprecipitate carries higher risks of pathogen transmission. Pathogen inactivation (PI) improves the safety of cryoprecipitate, but its effects on hemostatic properties remain unclear. This study investigated protein expression in samples of pathogen inactivated cryoprecipitate (PI-cryo) using non-targeted quantitative proteomics. Specifically, cryoprecipitate samples were reduced and alkylated and digested with trypsin. Approximately 50 micrograms of tryptic digests were analyzed by microflowLC-MS/MS. The LC separation used a Waters ACQUITY UPLC and a 1 mm x 150 mm 1.7 micron CSH C18 column using a flow rate of 100 microliters/min, a column temperature of 55C and a gradient of 3-28% (v/v) MeCN:H2O containing 0.1% formic acid over 60 minutes. The LC was interfaced to a Thermo Q-Exactive HF-X using the HESI-II source. The MS analysis used data-independent acquisition (DIA)-MS with a 120,000 resolution precursor ion (MS1) scan from 375-1500 m/z, AGC target of 3E6 and maximum injection time (IT) of 20 ms. DIA-MS/MS was performed using 30,000 resolution, AGC target of 3e6 and maximum IT of 60 ms, and 27 V NCE. The DIA windows included 15 x 37 m/z windows spanning 400-941 m/z with 1 m/z overlaps; as well as 3 x 87 m/z windows spanning 941-1200 m/z. Data was analyzed using Spectronaut Pulsar X
HostingRepository	MassIVE
AnnounceDate	2020-05-19
AnnouncementXML	Submission_2020-05-19_07:01:20.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matt Foster
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	instrument model

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-04 21:07:31	ID requested
⏵ 1	2020-05-19 07:01:20	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: plasma, apheresis, cryoprecipiate, microflow, data-independent acquisition

submitter keyword: plasma, apheresis, cryoprecipiate, microflow, data-independent acquisition

Contact List

Reed Kamyszek
contact affiliation	University of Michigan
contact email	rekamysz@med.umich.edu
lab head
Matthew W. Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
lab head
Matt Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085374/