PXD018978 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Investigating the protein-protein interactions of EccA3 in Mycobacterium smegmatis |
Description | Mycobacterium smegmatis encodes three ESX secretion systems, ESX-1, -3 and -4. Previous research has suggested localisation of the ESX-1 secretion system at the growing polar region of M. smegmatis. In a seperate study, we demonstrated unipolar localisation of EccA3 from ESX-3 at the growing polar region of M. smegmatis. Here we demonstrate that affinity purification of N-terminally FLAG-tagged MSMEG_0615 (EccA3) followed by mass spectrometry resulted in the co-purification proteins previously identified in the membrane fractions of M. tuberculosis. Furthermore, we demonstrate that EccA3 co-purified with proteins known to localise at the growing polar region of asymmetrically growing M. smegmatis, including Wag31 and CwsA. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-09 |
AnnouncementXML | Submission_2022-06-09_02:22:57.173.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nastassja Kriel |
SpeciesList | scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-04 16:27:39 | ID requested | |
⏵ 1 | 2022-06-09 02:22:58 | announced | |
Publication List
Kriel NL, Newton-Foot M, Bennion OT, Aldridge BB, Mehaffy C, Belisle JT, Walzl G, Warren RM, Sampson SL, Gey van Pittius NC, at the growing pole in Mycobacterium smegmatis. BMC Microbiol, 22(1):140(2022) [pubmed] |
Keyword List
submitter keyword: Mycobacterium smegmatis, AP-MS, ESX, polar localisation |
Contact List
Samantha Sampson |
contact affiliation | Department of Biomedical Sciences, Division of Molecular Biology and Human Genetics, Stellenbosch University, Cape Town, South Africa. |
contact email | ssampson@sun.ac.za |
lab head | |
Nastassja Kriel |
contact affiliation | Stellenbosch Univeristy |
contact email | nastassja@sun.ac.za |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD018978 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018978
- Label: PRIDE project
- Name: Investigating the protein-protein interactions of EccA3 in Mycobacterium smegmatis