PXD018967-1

PXD018967 is an original dataset announced via ProteomeXchange.

Title	CreA phosphorylation sites are important for the regulation of carbon catabolite repression in filamentous fungi
Description	Saprobic microorganisms, such as filamentous fungi of the genus Aspergillus, are of particular interest for biotechnological applications due to their natural capacity to secrete plant cell wall-degrading enzymes that are known as carbohydrate-active enzymes (CAZy). The presence of easily metabolizable sugars such as glucose, whose concentrations increase during plant biomass hydrolysis, results in the repression of CAZy-encoding genes, in a process known as carbon catabolite repression (CCR), which is undesired for the purpose of large-scale enzyme production. To date, the C2H2 transcription factor CreA has been described as the major CC-repressor in Aspergillus spp. Although CreA-mediated CCR has been extensively studied, less is known about the regulation of CreA itself and the role of post-translational modifications in this process. In this work, phosphorylation sites were identified by mass spectrometry on A. nidulans CreA and subsequently four sites, S262, S268, T308 and S319, were chosen to be mutated to non-phosphorylatable residues before their effects on CCR in both CC-repressing and de-repressing conditions were studied. Sites S262, S268 and T308 are important for CreA protein accumulation and cellular localization and repression of enzyme activities. In contrast, site S319 is key for CreA degradation and induction of enzyme activities. All sites were shown to be important for glycogen and trehalose metabolism. This study highlights the importance of CreA phosphorylation sites for the regulation of CCR. Furthermore, these sites are interesting targets for biotechnological strain engineering without the need to delete essential genes which can result in undesired side effects.
HostingRepository	PRIDE
AnnounceDate	2021-02-18
AnnouncementXML	Submission_2021-02-18_08:11:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Aspergillus nidulans; NCBI TaxID: 162425;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-04 01:26:22	ID requested
⏵ 1	2021-02-18 08:11:08	announced
2	2023-11-14 08:51:03	announced	2023-11-14: Updated project metadata.

10.1128/MBIO.03146-20;

submitter keyword: carbon catabolite repression, Aspergillus nidulans, CreA, LC-MS, phosphoproteomics

Olaf Kniemeyer
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute Departement of Molecular and Applied Microbiology Germany
contact email	olaf.kniemeyer@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018967
     1. Label: PRIDE project
     2. Name: CreA phosphorylation sites are important for the regulation of carbon catabolite repression in filamentous fungi