PXD018965-1

PXD018965 is an original dataset announced via ProteomeXchange.

Title	TrypOx, a novel eukaryotic homologue of the redox-regulated chaperone Hsp33 in Trypanosoma brucei
Description	In this study, we describe a novel eukaryotic homologue of Hsp33 in Trypanosoma brucei.Bioinformatics analysis of the Hsp33 family suggested that eukaryotic pathogens belonging to the kinetoplastids, such as Trypanosoma cruzi,Trypanosoma brucei spp, and leishmaniasis had persevered Hsp33 homologues in their genomes. We showed that RNAi silencing of TrypOx led to a significant decrease in the survival of T.brucei under mild oxidative stress conditions,and then we adopted a proteomics-driven approach to investigatethe role of TrypOx in defining the oxidative stress response. Our results showed that depletion of TrypOx significantly altered the abundance of proteins mediating redox homeostasis,linking TrypOx with the antioxidant system and implying a protetcive role of Trypox against oxidative stress.
HostingRepository	PRIDE
AnnounceDate	2020-07-28
AnnouncementXML	Submission_2020-07-27_23:52:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samar Aramin
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-04 01:26:03	ID requested
⏵ 1	2020-07-27 23:52:42	announced
2	2024-10-22 05:09:45	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Trypanosoma brucei, LC-MS/MS

Dana Reichmann
contact affiliation	Dept. of Biological Chemistry,The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem,Givat Ram Campus, Jerusalem, 91904, Israel
contact email	danare@mail.huji.ac.il
lab head
Samar Aramin
contact affiliation	Hebrew University
contact email	samar.aramin@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018965
     1. Label: PRIDE project
     2. Name: TrypOx, a novel eukaryotic homologue of the redox-regulated chaperone Hsp33 in Trypanosoma brucei