PXD018964-2

PXD018964 is an original dataset announced via ProteomeXchange.

Title	Integrated analysis of sialotranscriptome and sialoproteome of the brown dog tick Rhipicephalus sanguineus (s.l.): insights into gene expression during blood feeding
Description	Tick salivary glands secrete a complex saliva into their hosts which modulates vertebrate hemostasis, immunity and tissue repair mechanisms. In this work, we explored using transcriptomic and proteomic analysis the sialome of Rhipicephalus sanguineus (s.l.) adult female ticks feeding on a rabbit. Instead of grouping the ticks by days of feeding, we instead grouped them into six groups based on their weights which better demarks their feeding physiological state. We also included an unfed group. All groups had three replicates except for the unfed group that had two replicates. A total of 687 million reads from 20 libraries were assembled from which 71,643 coding sequences were extracted, 28,921 of which had a TPM (transcripts per million) of 10 or larger in at least one library. The translated transcripts served as a target database for a proteomic study, which identified 2,125 polypeptides. The correlations between transcriptional and translational results in the different groups were evaluated.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:21:37.927.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lucas Tirloni
SpeciesList	scientific name: Rhipicephalus sanguineus complex sp. ME-2011; NCBI TaxID: 943274;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-04 01:26:00	ID requested
1	2021-05-03 07:27:46	announced
⏵ 2	2024-10-22 05:21:38	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: tick: saliva: proteome: parasite

Jose M. C. Ribeiro
contact affiliation	Laboratory of Malaria and Vector Research, NIAID, NIH
contact email	JRIBEIRO@niaid.nih.gov
lab head
Lucas Tirloni
contact affiliation	Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health
contact email	lucas.tirloni@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD018964

PRIDE project URI

• PRIDE
  1. PXD018964
     1. Label: PRIDE project
     2. Name: Integrated analysis of sialotranscriptome and sialoproteome of the brown dog tick Rhipicephalus sanguineus (s.l.): insights into gene expression during blood feeding