PXD018942 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Syndecan-2 partners in rat left ventricular lysates |
Description | To get more insight into the role of syndecan-2, we here sought to identify interaction partners of syndecan-2 in rat left ventricle. By using three different affinity purification methods combined with mass spectrometry (AP-MS) analysis, we identified 30 novel partners and 9 partners previously described in the literature, which together make up the first cardiac syndecan-2 interactome. The three AP-MS were: 1) Immunoprecipitation of endogenous SDC2. Non-relevant IgG was used as negative control. 2) Fishing with recombinant GST-SDC2 (full length). GST and beads only were used as negative controls. 3) Pull down using a synthetic biotin-axh-SDC2cyt peptide (cytoplasmic tail). A scrambled peptide sequence and beads only were used as negative controls. |
HostingRepository | PRIDE |
AnnounceDate | 2020-12-01 |
AnnouncementXML | Submission_2020-12-01_03:12:23.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tuula Nyman |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-30 16:46:41 | ID requested | |
⏵ 1 | 2020-12-01 03:12:24 | announced | |
Publication List
Mathiesen SB, Lunde M, Stensland M, Martinsen M, Nyman TA, Christensen G, Carlson CR, The Cardiac Syndecan-2 Interactome. Front Cell Dev Biol, 8():792(2020) [pubmed] |
Keyword List
submitter keyword: Syndecan-2, interactome, mass spectrometry |
Contact List
Tuula Nyman |
contact affiliation | Head of Proteomics |
contact email | tuula.nyman@medisin.uio.no |
lab head | |
Tuula Nyman |
contact affiliation | University of Oslo |
contact email | tuula.nyman@medisin.uio.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD018942 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018942
- Label: PRIDE project
- Name: Syndecan-2 partners in rat left ventricular lysates