PXD018899 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FAK activity in cancer-associated fibroblasts is a prognostic marker and a druggable key metastatic player in pancreatic cancer |
Description | Cancer-associated fibroblasts (CAFs), although considered as the most abundant pancreatic ductal adenocarcinoma (PDAC) stromal cells playing a critical role in tumor progression and chemoresistance, are not yet directly druggable. Here we report that focal adhesion kinase (FAK) activity (evaluated based on 397 tyrosine phosphorylation level) in CAFs is highly increased compared to its activity in fibroblasts from healthy pancreas. Fibroblastic FAK activity is an independent prognostic marker for disease free and overall survival of PDAC patients (cohort of 120 PDAC samples). Genetic inactivation of FAK within fibroblasts (FAK-kinase-dead, KD) reduces fibrosis and immunosuppressive cell number within primary tumor and dramatically decreases tumor spread. Mechanistically, pharmacologic and genetic fibroblastic FAK inactivation reduces fibroblast migration/invasion, decreases extracellular matrix (ECM) expression and deposition by CAFs, and negatively impacts M2 macrophage polarization and migration. Thus, FAK activity within CAFs appears as an independent PDAC prognostic marker and a druggable driver of tumor cell invasion. |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-19 |
AnnouncementXML | Submission_2020-08-19_05:55:17.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Vinothini Rajeeve |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-29 05:06:00 | ID requested | |
⏵ 1 | 2020-08-19 05:55:18 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: FAK, extracellular matrix, cancer-associated fibroblasts, pancreatic cancer |
Contact List
Vinothini Rajeeve, Pedro Cutillas |
contact affiliation | Reader in Centre for Genomics and Computational Biology Barts Cancer Institute Queen Mary, University of London John Vane Science Centre, Charterhouse Square, London EC1M 6BQ Tel: +44 (0)20 7882 8266 | Fax: +44 (0)20 7882 3891 | Email: p.cutillas@qmul.ac.uk www.bci.qmul.ac.uk/research/centre-profiles/haemato-oncology.html https://www.bci.qmul.ac.uk/en/staff/item/pedro-cutillas |
contact email | p.cutillas@qmul.ac.uk |
lab head | |
Vinothini Rajeeve |
contact affiliation | Centre for Haemato Oncology |
contact email | v.rajeeve@qmul.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018899
- Label: PRIDE project
- Name: FAK activity in cancer-associated fibroblasts is a prognostic marker and a druggable key metastatic player in pancreatic cancer