PXD018881-1

PXD018881 is an original dataset announced via ProteomeXchange.

Title	ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response
Description	Mitochondria are tightly embedded within metabolic and regulatory networks that optimize plant performance in response to environmental challenges. The best-known mitochondrial retrograde signaling pathway involves stress-induced activation of the transcription factor NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), which initiates protective responses to stress-induced mitochondrial dysfunction in Arabidopsis (Arabidopsis thaliana). Post-translational control of the elicited responses, however, remains poorly understood. Previous studies linked protein phosphatase 2A subunit PP2A-B’γ, a key negative regulator of stress responses, with reversible phosphorylation of ACONITASE 3 (ACO3). Here we report on ACO3 and its phosphorylation at Ser91 as key components of stress regulation that are induced by mitochondrial dysfunction. Targeted mass spectrometry-based proteomics revealed that the abundance and phosphorylation of ACO3 increased under stress, which required signaling through ANAC017. Phosphomimetic mutation at ACO3-Ser91 and accumulation of ACO3S91D-YFP promoted the expression of genes related to mitochondrial dysfunction. Furthermore, ACO3 contributed to plant tolerance against UV-B or antimycin A-induced mitochondrial dysfunction. These findings demonstrate that ACO3 is both a target and mediator of mitochondrial dysfunction signaling, and critical for achieving stress tolerance in Arabidopsis leaves.
HostingRepository	PanoramaPublic
AnnounceDate	2021-05-14
AnnouncementXML	Submission_2021-05-14_07:16:51.329.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jesús Pascual
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	Phospho; Carbamidomethyl
Instrument	Q Exactive; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-28 19:59:35	ID requested
⏵ 1	2021-05-14 07:16:51	announced
2	2022-03-14 21:15:33	announced	: Added PubMed Id

Pascual J, Rahikainen M, Angeleri M, Alegre S, Gossens R, Shapiguzov A, Heinonen A, Trotta A, Durian G, Winter Z, Sinkkonen J, Kangasjärvi J, Whelan J, Kangasjärvi S. ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response. Plant Physiol [Internet]. 2021 May 12

Available from: https://doi.org/10.1093/plphys/kiab225

submitter keyword: aconitase, mitochondrial dysfunction response, phosphorylation, post-translational regulation

Saijaliisa Kangasjärvi
contact affiliation	Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki
contact email	saijaliisa.kangasjärvi@helsinki.fi
lab head
Jesús Pascual
contact affiliation	Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland
contact email	jepavaz@utu.fi
dataset submitter

Panorama Public dataset URI