PXD018868-1

PXD018868 is an original dataset announced via ProteomeXchange.

Title	Proteome responses of Grapevine to Downy mildew
Description	A comparative proteomic analysis of grapevine leaves from the resistant genotype V. davidii ‘LiuBa-8’ (LB) and susceptible genotype V. vinifera ‘Pinot Noir’ (PN) at 12 hpi was conducted to understand the complex relationship between grapevine and P. viticola and the molecular mechanisms between difference resistant vitis genotypes at the early stage of infection. A total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN respectively at 12 hpi by iTRAQ. MapMan analysis showed that the majority of these DEPs were related to photosynthesis, metabolism, stress and redox. More up-expressed DEPs involved in photosynthesis and less down-expressed DEPs involved in metabolism contribute to the resistance in LB. PR10.2, PR10.3, HSF70.2 and HSP90.6 are proposed to be key proteins in both compatible and incompatible interactions. Accumulation H2O2 established the ROS signaling in incompatible interaction and APXs, GSTs maybe associated with the resistance of grapevine against downy mildew. Moreover, we verified four proteins to ensure the accuracy of proteome data using PRM. Overall, these data provide new insights into molecular events and provide valuable candidate proteins that could be used to illuminate molecular mechanisms underlying the incompatible and compatible interaction in early stage and eventually to be exploited to develop new protection strategy against downy mildew in grapevine.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_10:55:26.007.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guotian Liu
SpeciesList	scientific name: Vitis vinifera (Grape); NCBI TaxID: 29760;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-28 10:27:15	ID requested
⏵ 1	2022-02-15 10:55:26	announced

Liu GT, Wang BB, Lecourieux D, Li MJ, Liu MB, Liu RQ, Shang BX, Yin X, Wang LJ, Lecourieux F, Xu Y, Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola. Hortic Res, 8(1):100(2021) [pubmed]

submitter keyword: grapevine, P.viticola, iTRAQ, compatible and incompatible interactions

Yan Xu
contact affiliation	College of Horticulture, Northwest A&F University
contact email	yan.xu@nwafu.edu.cn
lab head
Guotian Liu
contact affiliation	Northwest A&F University
contact email	liuguotian555@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018868

PRIDE project URI

• PRIDE
  1. PXD018868
     1. Label: PRIDE project
     2. Name: Proteome responses of Grapevine to Downy mildew