PXD018865-1

PXD018865 is an original dataset announced via ProteomeXchange.

Title	Mouse Muscle Stem Cell Proteome during Activation and CPEB1 Dependent Proteome in Mouse Muscle Stem Cell
Description	Muscle Stem Cells or satellite cells (SCs) are required for muscle regeneration. In resting muscles, SCs are kept in quiescence. After injury, SCs undergo rapid activation, proliferation and differentiation to repair damaged muscles. The transcriptome alteration during SC activation is well characterized. While transcriptome is not exactly represent proteome because of post-transcriptional regulations such as miRNA induced gene silencing. However, little is known about SC proteome. We obtained quisecent SCs (QSCs) and freshly isolated SCs (fiSCs, early activated SCs) and activated SCs (ASCs) for high resolution mass spectrometry Bruker timsTOF Pro. Thus, we uncovered the QSC proteome. By comparison of QSC proteome to fiuSCs proteome or ASCs proteome, we identified the pathways that are differentially expressed between them. Forthermore, we characterized a translational regulator CPEB1 reprogrammed translation landscape for SC activation.
HostingRepository	PRIDE
AnnounceDate	2021-11-05
AnnouncementXML	Submission_2021-11-05_04:07:18.831.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018865
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tom Cheung
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Phospho; Deamidated; Oxidation; Acetyl; Carbamidomethyl
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-28 09:30:13	ID requested
⏵ 1	2021-11-05 04:07:19	announced

Dataset with its publication pending

submitter keyword: Mouse, Muscle Stem Cell, CPEB1, Proteome, Activation

Tom. H. CHEUNG
contact affiliation	Division of Life Science, Center for Stem Cell Research, Center of Systems Biology and Human Health, State Key Laboratory in Molecular Neuroscience, Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. Guangdong Provincial Key Laboratory of Brain Science, Disease and Drug Development, HKUST Shenzhen Research Institute, Shenzhen-Hong Kong Institute of Brain Science, 518057, Shenzhen, Guangdong, China.
contact email	tcheung@ust.hk
lab head
Tom Cheung
contact affiliation	Hong Kong University of Science and Technology
contact email	tcheung@ust.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018865
     1. Label: PRIDE project
     2. Name: Mouse Muscle Stem Cell Proteome during Activation and CPEB1 Dependent Proteome in Mouse Muscle Stem Cell