PXD018857 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ubiquitin-mediated modulation of necroptotic effector MLKL |
Description | We aimed to identify lysine (K) residues that are endogenously ubiquitylated upon TNF-induced necroptosis. To this end, we used a quantitative mass spectrometry-based approach. Briefly, MDFs were treated with TSZ or left untreated and protein lysates were digested with trypsin into peptides. Ubiquitylated peptides bearing the di-glycine (K-GG) remnant were enriched by immunoprecipitation with anti-K-ε-GG antibody. A total of three biological replicates were prepared and the 6 samples were labelled with Tandem Mass Tags (TMT). Samples were mixed and analysing by Liquid Chromatography-Tandem Mass Spectroscopy (LC-MS). Quantification was performed to determine the relative abundance of site-specific ubiquitylation events in each condition. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-13 |
AnnouncementXML | Submission_2021-04-13_01:56:06.723.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | James Wright |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; geranylgeranylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-28 08:22:27 | ID requested | |
⏵ 1 | 2021-04-13 01:56:07 | announced | |
2 | 2021-07-19 23:29:38 | announced | 2021-07-20: Updated publication reference for PubMed record(s): 34099649. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Ubiquitin, MLKL, Cancer, Mouse, MSMS, TMT |
Contact List
Jyoti Choudhary |
contact affiliation | The Institute of Cancer Research, London |
contact email | jyoti.choudhary@icr.ac.uk |
lab head | |
James Wright |
contact affiliation | The Institute of Cancer Research |
contact email | james.wright@icr.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018857
- Label: PRIDE project
- Name: Ubiquitin-mediated modulation of necroptotic effector MLKL