PXD018834-1

PXD018834 is an original dataset announced via ProteomeXchange.

Title	Chaperonin CCT8 is essential for T cell development and function
Description	T cells rely for their development and function on the correct folding and turnover of proteins generated in response to a broad range of molecular cues. Here we demonstrate that the eukaryotic type II chaperonin complex CCT is indispensable for T cell development, function, stress-response, and metabolism. CCT-deficient thymoyctes are compromised in their selection, differentiation, and late stage maturation. The lack of CCT expression prevents a normal response to T cell activation induced changes in the proteome, stops the formation of nuclear actin filaments and impairs a normal cells stress response. Consequently, T cell homeostatic maintenance and receptor-mediated stimulation of proliferation are impaired in CCT-deficient T cells leading to activation-induced cell death. Furthermore, Th2 polarization digresses in the absence of CCT-controlled protein folding leading paradoxically to continued IFN-expression. As a result, CCT-deficient T cells fail to generate an efficient immune protection against helminths as they are unable to sustain a coordinated recruitment of the innate and adaptive immune systems. Taken together, these findings demonstrate that both normal thymopoiesis and peripheral T cell biology are critically dependent on CCT-controlled proteostasis and that its absence is incompatible with protective immunity.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_23:44:40.745.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simon Davis
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-27 08:17:22	ID requested
⏵ 1	2021-09-08 23:44:41	announced
2	2024-10-22 05:27:54	announced	2024-10-22: Updated project metadata.

Oftedal BE, Maio S, Handel AE, White MPJ, Howie D, Davis S, Prevot N, Rota IA, Deadman ME, Kessler BM, Fischer R, Trede NS, Sezgin E, Maizels RM, Holl, ä, nder GA, The chaperonin CCT8 controls proteostasis essential for T cell maturation, selection, and function. Commun Biol, 4(1):681(2021) [pubmed]

submitter keyword: Mouse, T cell, T cell development, LC-MS/MS

Roman Fischer
contact affiliation	Target Discovery Institute (TDI), Nuffield Department of Medicine, University of Oxford, United Kingdom
contact email	roman.fischer@ndm.ox.ac.uk
lab head
Simon Davis
contact affiliation	University of Oxford
contact email	simon.davis2@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018834
     1. Label: PRIDE project
     2. Name: Chaperonin CCT8 is essential for T cell development and function