PXD018826-1

PXD018826 is an original dataset announced via ProteomeXchange.

Title	Hemolymph proteome of the pea aphid Acyrthosiphon pisum
Description	The pea aphid, Acyrthosiphon pisum, can host different facultative symbionts (FS), which may provide various benefits to the host, including adaptation to the host plant and resistance to heat or natural enemies (fungi, bacteria, parasitoid wasps). Here, we searched whether and how the presence of some FS could affect a key component of insect innate immunity, the phenoloxidase, under normal and stressed conditions. For this, we used A. pisum clones of different genetic background (LL01, YR2 and T3-8V1) and harboring or not FS (Regiella insecticola (Ri), Hamiltonella defensa (Hd) or Serratia symbiotica (Ss)). Proteomic analysis of aphid hemolymph and PCR indicated that the two A. pisum phenoloxidases, PO1 and PO2, are expressed and translated into protein. They seem mainly secreted as circulating enzymes in the hemolymph and a proteolytic cleavage was not necessary for their activation. PO genes expression was dependent upon the aphid genotypes as well as the amount of PO proteins and activity in the total hemolymph (T3-8V1-Amp > LL01 = YR2-Amp). The presence in YR2 and T3-8V1 clones of Hd or Ri, but not Ss, caused a sharp decrease in PO activity by interfering with both transcription and translation. Microinjection of different types of stressors (yeast, E. coli, latex beads) in YR2 lines affected the survival rate of aphids and in most cases, it also decreases the PO genes expression after 24h, whereas the amount and activity of the proteins varied differently depending on the FS and the stressor, regardless of the genes expression. These data provide new hypothesis on the mechanism by which some facultative symbionts act on the pea aphid immunity.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:40:54.033.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018826
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maya BELGHAZI
SpeciesList	scientific name: Acyrthosiphon pisum (Pea aphid); NCBI TaxID: 7029;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-27 02:55:29	ID requested
⏵ 1	2024-10-07 10:40:54	announced

10.6019/PXD018826;

Luo C, Belghazi M, Schmitz A, Lemauf S, Desneux N, Simon JC, Poiri, é M, Gatti JL, Hosting certain facultative symbionts modulates the phenoloxidase activity and immune response of the pea aphid Acyrthosiphon pisum. Insect Sci, 28(6):1780-1799(2021) [pubmed]

10.1111/1744-7917.12888;

submitter keyword: Phenoloxidases, stressors, Acyrthosiphon pisum, Regiella insecticola, Hemolymph, Facultative symbionts, Serratia symbiotica, Hamiltonella defensa,Pea aphid

Jean Luc GATTI
contact affiliation	Evolution and Specificity of Multitrophic Interactions (ESIM) Sophia Agrobiotech Institute (ISA) INRAE / CNRS/ Université de Nice 400 route des Chappes BP 167 06903 Sophia Antipolis Cedex - France
contact email	Jean-Luc.gatti@inrae.fr
lab head
Maya BELGHAZI
contact affiliation	CNRS
contact email	maya.belghazi@univ-amu.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD018826

PRIDE project URI

• PRIDE
  1. PXD018826
     1. Label: PRIDE project
     2. Name: Hemolymph proteome of the pea aphid Acyrthosiphon pisum