PXD018810-1

PXD018810 is an original dataset announced via ProteomeXchange.

Title	Thermal proteome profiling of Toxoplasma gondii lysates treated with enhancer 1 (ENH1)
Description	Previously, we characterized a chemical modulator, ENH1, that exhibited potent antiparasitic properties against apicomplexans and induced calcium oscillations, but the target eluded identification. To determine the target of ENH1, we employed thermal proteome profiling in parasites. We treated extracellular parasites with vehicle (DMSO) or ENH1 for 15 minutes at 37°C, under conditions previously determined to induce calcium fluxes in T. gondii.8 Parasites were then heated at 10 different temperatures to induce thermal denaturation. Following lysis and high-speed centrifugation, soluble protein was isolated and analyzed by mass spectrometry to generate thermal stability profiles from each condition across two biological replicates.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:12:04.556.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018810
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alice Herneisen
SpeciesList	scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationList	Biotin:Sigma-B1267; TMT6plex-126 reporter+balance reagent acylated residue; Phospho; Methylthio; methylthiolated residue; Label:13C(6)15N(4); TMT6plex; phosphorylated residue; Oxidation; isotope labeled residue; Acetyl; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-26 14:52:15	ID requested
⏵ 1	2024-10-08 11:12:09	announced

Herneisen AL, Sidik SM, Markus BM, Drewry DH, Zuercher WJ, Lourido S, Using Thermal Proteome Profiling. ACS Chem Biol, 15(7):1801-1807(2020) [pubmed]

10.6019/PXD018810;

10.1021/acschembio.0c00369;

submitter keyword: thermal proteome profiling,Toxoplasma gondii, CETSA

Sebastian Lourido
contact affiliation	Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Biology, Cambridge, MA, USA
contact email	lourido@wi.mit.edu
lab head
Alice Herneisen
contact affiliation	Whitehead Institute for Biomedical Research, Cambridge, MA, USA 02142 Department of Biology, Massachusetts Insitute of Technology
contact email	alice@wi.mit.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018810
     1. Label: PRIDE project
     2. Name: Thermal proteome profiling of Toxoplasma gondii lysates treated with enhancer 1 (ENH1)