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PXD018810-1

PXD018810 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThermal proteome profiling of Toxoplasma gondii lysates treated with enhancer 1 (ENH1)
DescriptionPreviously, we characterized a chemical modulator, ENH1, that exhibited potent antiparasitic properties against apicomplexans and induced calcium oscillations, but the target eluded identification. To determine the target of ENH1, we employed thermal proteome profiling in parasites. We treated extracellular parasites with vehicle (DMSO) or ENH1 for 15 minutes at 37°C, under conditions previously determined to induce calcium fluxes in T. gondii.8 Parasites were then heated at 10 different temperatures to induce thermal denaturation. Following lysis and high-speed centrifugation, soluble protein was isolated and analyzed by mass spectrometry to generate thermal stability profiles from each condition across two biological replicates.
HostingRepositoryPRIDE
AnnounceDate2024-10-08
AnnouncementXMLSubmission_2024-10-08_11:12:04.556.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD018810
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlice Herneisen
SpeciesList scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationListBiotin:Sigma-B1267; TMT6plex-126 reporter+balance reagent acylated residue; Phospho; Methylthio; methylthiolated residue; Label:13C(6)15N(4); TMT6plex; phosphorylated residue; Oxidation; isotope labeled residue; Acetyl; monohydroxylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-26 14:52:15ID requested
12024-10-08 11:12:09announced
Publication List
Herneisen AL, Sidik SM, Markus BM, Drewry DH, Zuercher WJ, Lourido S, Using Thermal Proteome Profiling. ACS Chem Biol, 15(7):1801-1807(2020) [pubmed]
10.6019/PXD018810;
10.1021/acschembio.0c00369;
Keyword List
submitter keyword: thermal proteome profiling,Toxoplasma gondii, CETSA
Contact List
Sebastian Lourido
contact affiliationWhitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Biology, Cambridge, MA, USA
contact emaillourido@wi.mit.edu
lab head
Alice Herneisen
contact affiliationWhitehead Institute for Biomedical Research, Cambridge, MA, USA 02142 Department of Biology, Massachusetts Insitute of Technology
contact emailalice@wi.mit.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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