PXD018772 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dissecting the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor |
Description | In a number of species, individuals exposed to pathogens can mount an immune response and transmit this immunological experience to their offspring, thereby protecting them against persistent threats. Such vertical transfer of immunity, named trans-generational immune priming (TGIP), has been described in both vertebrates and invertebrates. Although increasingly studied during the last decade, the mechanisms underlying TGIP in invertebrates are still elusive, especially those protecting the earliest offspring life stage, i.e. the embryo developing in the egg. In the present study, we took the mealworm beetle Tenebrio molitor as a biological model, and combined different proteomic and transcriptomic approaches to determine whether mothers transfer a “signal” (such as fragments of infecting bacteria), mRNA and/or protein/peptide effectors to protect their eggs against two natural bacterial pathogens, namely the Gram-positive Bacillus thuringiensis (Bt) and the Gram-negative Serratia entomophila (Se). We demonstrated that eggs are mainly protected by an active direct transfer of a restricted number of immune proteins and of antimicrobial peptides. In contrast, the present data clearly reject the involvement of mRNA transfer while the transmission of a “signal”, if it happens, is marginal and only occurs within 24h after maternal exposure to bacteria. This work exemplifies the power of combining global multidisciplinary approaches to disentangle the different scenarios of a complex trait, providing a comprehensive characterization of TGIP mechanisms in T. molitor. It also paves the way for future alike studies focusing on TGIP in a wide range of invertebrates and vertebrates to identify additional candidates that could be specific to TGIP and to investigate whether the TGIP mechanisms found herein are specific or common to all insect species. |
HostingRepository | PRIDE |
AnnounceDate | 2020-10-08 |
AnnouncementXML | Submission_2020-10-08_00:00:46.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sebastien Voisin |
SpeciesList | scientific name: Tenebrio molitor; NCBI TaxID: 7067; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-24 01:17:59 | ID requested | |
⏵ 1 | 2020-10-08 00:00:47 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Trans-generational immune priming |
invertebrate immunity |
host-pathogens interaction |
molecular mechanisms |
Omics |
antimicrobial peptides |
Contact List
Philippe Bulet |
contact affiliation | CR Université Grenoble Alpes, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, La Tronche, France. Plateforme BioPark d'Archamps, ArchParc, Saint Julien en Genevois, France |
contact email | philippe.bulet@univ-grenoble-alpes.fr |
lab head | |
Sebastien Voisin |
contact affiliation | Plateforme BioPark of Archamps |
contact email | sebastien.voisin@biopark-archamps.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD018772 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018772
- Label: PRIDE project
- Name: Dissecting the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor