PXD018710 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dynamic Palmitoylation Events Following T-Cell Receptor Signaling |
Description | Palmitoylation is the reversible addition of palmitate to cysteine via a thioester linkage. The reversible nature of this modification makes it a prime candidate as a mechanism for regulating signal transduction in T-cell receptor signaling. Following stimulation of the T-cell receptor we find a number of proteins are newly palmitoylated, including those involved in vesicle-mediated transport and Ras signal transduction. Among these stimulation-dependent palmitoylation targets are the v-SNARE VAMP7, important for docking of vesicular LAT during TCR signaling, and the largely undescribed palmitoyl acyltransferase DHHC18 that is expressed in two isoforms in T cells. Using our newly developed On-Plate Palmitoylation Assay (OPPA), we show DHHC18 is capable of palmitoylating VAMP7 at Cys183. Cellular imaging shows that the palmitoylation-deficient protein fails to be retained at the Golgi. This dataset includes the experiments previously reported in: Morrison et al. Quantitative analysis of the human T cell palmitome. Sci Rep. 5, Article number: 11598 (2015) |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-08_18:52:45.041.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Miguel Alvaro |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-21 05:34:22 | ID requested | |
⏵ 1 | 2021-09-08 18:52:45 | announced | |
Publication List
Morrison E, Wegner T, Zucchetti AE, Á, lvaro-Benito M, Zheng A, Kliche S, Krause E, Br, ü, gger B, Hivroz C, Freund C, Dynamic palmitoylation events following T-cell receptor signaling. Commun Biol, 3(1):368(2020) [pubmed] |
Keyword List
submitter keyword: Palmitoylation, S-acylation, TCR Signaling, T-Cell Activation, Palmitome, In vitro palmitoylation, VAMP7, Ti-VAMP, LAT, DHHC PATs, DHHC18, DHHC20 |
Contact List
Christian Freund |
contact affiliation | Freie Universität Berlin, Institut für Chemie und Biochemie, Thielallee 63, Berlin, 14195, Germany |
contact email | chfreund@zedat.fu-berlin.de |
lab head | |
Miguel Alvaro |
contact affiliation | FU Berlin |
contact email | malvaro@zedat.fu-berlin.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018710
- Label: PRIDE project
- Name: Dynamic Palmitoylation Events Following T-Cell Receptor Signaling