PXD018600-1

PXD018600 is an original dataset announced via ProteomeXchange.

Title	A Structure-Based Mechanism for DNA Entry into the Cohesin Ring
Description	The ring-shaped cohesin complex is thought to fulfil its roles in sister chromatid cohesion, genome stability and gene regulation by topologically encircling DNAs. The ring is formed by two Structural Maintenance of Chromosome (SMC) subunits, whose ATPase heads are linked by a kleisin subunit. Additional components, including the Mis4Scc2/NIPL cohesin loader, engage the kleisin. Here, we visualize a DNA gripping intermediate during cohesin loading onto DNA by cryo-electron microscopy. ATP-dependent head engagement creates an interaction surface onto which Mis4Scc2/NIPL clamps the DNA. We use biophysical tools to establish the order of events during cohesin loading. DNA first traverses an N-terminal kleisin gate that was opened by ATP binding and closed again as the loader locks the DNA. Ensuing ATP hydrolysis and head disengagement, assisted by Mis4Scc2/NIPL, will complete DNA entry. A conserved kleisin N-terminal tail guides the DNA on its trajectory to successful topological DNA entry into the cohesin ring.
HostingRepository	PRIDE
AnnounceDate	2020-08-05
AnnouncementXML	Submission_2020-08-04_23:17:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018600
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Helen Flynn
SpeciesList	scientific name: Schizosaccharomyces pombe 972h-; NCBI TaxID: 284812;
ModificationList	Oxidation; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-16 05:36:14	ID requested
⏵ 1	2020-08-04 23:17:34	announced

Dataset with its publication pending

submitter keyword: Chromosome segregation, Sister chromatid cohesion, SMC complexes, Cohesin, ABC-ATPase, Mis4/Scc2/NIPBL, Cryo-electron microscopy, DNA-protein crosslink mass spectrometry, S. pombe

Helen Flynn
contact affiliation	Mass Spectrometry Proteomics Science Technology Platform The Francis Crick Institute 1 Midland Road London United Kingdom
contact email	bram.snijders@crick.ac.uk
lab head
Helen Flynn
contact affiliation	The Francis Crick Institute
contact email	helen.flynn@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018600
     1. Label: PRIDE project
     2. Name: A Structure-Based Mechanism for DNA Entry into the Cohesin Ring