PXD018592-3

PXD018592 is an original dataset announced via ProteomeXchange.

Title	IL-22 promotes postnatal formation of MUC17 glycocalyx barrier that protects host enterocytes
Description	The apical aspects of absorptive epithelial cells form a highly organized brush border membrane (BBM), shaped by densely packed microvilli that are coated with a glycocalyx. Here, we present evidence showing that the glycocalyx forms an epithelial barrier that prevents exogenous molecules from gaining access to the BBM. We used a multiomics approach to investigate function and regulation of membrane mucins exposed on the enterocytic BBM during postnatal development of the small intestine. Muc17 was identified as a major membrane mucinin the glycocalyx that was specifically upregulated by IL-22 as part of an epithelial defense repertoire during suckling-weaning transition. High levels of IL-22 at the time of weaning primed progenitor enterocyte to express Muc17 as they migrate out of intestinal crypts to replace neonatal enterocytes. Our findings propose a role for membrane mucin Muc17 in epithelial barrier function in the small intestine.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:18:59.053.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liisa Arike
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-15 15:55:34	ID requested
1	2021-02-16 02:25:57	announced
2	2021-09-28 03:51:00	announced	2021-09-28: Updated publication reference for PubMed record(s): 33596425.
⏵ 3	2024-10-22 05:19:01	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2021.108757;

Layunta E, J, ä, verfelt S, Dolan B, Arike L, Pelaseyed T, IL-22 promotes the formation of a MUC17 glycocalyx barrier in the postnatal small intestine during weaning. Cell Rep, 34(7):108757(2021) [pubmed]

submitter keyword: innate immunity, mucin, intestine, microvilli, enterocyte,MUC13, microbiota, MUC17, glycocalyx

Liisa Arike
contact affiliation	Mucin Biology Group, Proteomics Laboratory, Department of Medical Biochemistry and Cell Biology, University of Gothenburg
contact email	liisaarike@gmail.com
lab head
Liisa Arike
contact affiliation	PhD
contact email	liisaarike@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD018592

PRIDE project URI

• PRIDE
  1. PXD018592
     1. Label: PRIDE project
     2. Name: IL-22 promotes postnatal formation of MUC17 glycocalyx barrier that protects host enterocytes