PXD018531-1

PXD018531 is an original dataset announced via ProteomeXchange.

Title	DPAGT1-Mediated Protein N-Glycosylation Is Indispensable for Oocyte and Follicle Development in Mice
Description	Posttranslational modification of proteins by N-linked glycosylation is crucial for many life processes. However, the exact contribution of N-glycosylation to mammalian female reproduction remains largely undefined. Here, DPAGT1, the enzyme that catalyzes the first step of protein N-glycosyation, is identified to be indispensable for oocyte development in mice. A recessive missense mutation (c. 497A>G; p. Asp166Gly) of Dpagt1 causes female subfertility without grossly affecting other functions. Mutant females ovulate fewer eggs owing to defects of follicular development beyond the late secondary stage. Oocytes ovulated by mutants carry a thin and fragile zona pellucida, and display poor developmental competence after fertilization in vitro. Moreover, completion of the first meiosis is accelerated in mutant oocytes, which is coincident with the elevation of aneuploidy. Mechanistically, transcriptomic analysis reveals the downregulation of a number of transcripts essential for oocyte meiotic progression and preimplantation development (e.g., Pttgt1, Esco2, Orc6, and Npm2) in mutant oocytes, which could account for the defects observed. Furthermore, conditional knockout (CKO) of Dpagt1 in oocytes recapitulates the phenotypes observed in Dpagt1 mutant females, and causes complete infertility. Taken together, these data indicate that protein N-glycosylation in oocytes is essential for female fertility in mammals by specific control of oocyte development.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_00:32:44.730.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Li Hui
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	complex glycosylation
Instrument	6410 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-14 02:48:32	ID requested
⏵ 1	2021-09-09 00:32:45	announced
2	2024-10-22 05:28:14	announced	2024-10-22: Updated project metadata.

Li H, You L, Tian Y, Guo J, Fang X, Zhou C, Shi L, Su YQ, -Glycosylation Is Indispensable for Oocyte and Follicle Development in Mice. Adv Sci (Weinh), 7(14):2000531(2020) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Glycoproteomics (B/D-HPP)

submitter keyword: Mouse,Ovary,LC-MS/MS

Li Hui
contact affiliation	State Key Laboratory of Reproduction Medicine
contact email	3154237809@qq.com
lab head
Li Hui
contact affiliation	State Key Laboratory of Reproductive Medicine
contact email	3154237809@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018531
     1. Label: PRIDE project
     2. Name: DPAGT1-Mediated Protein N-Glycosylation Is Indispensable for Oocyte and Follicle Development in Mice