PXD018526-2

PXD018526 is an original dataset announced via ProteomeXchange.

Title	Analysis of the interactome of LAT in CRISPR/CAS9 modified mouse CD+ T cells
Description	Using a CRISPR/Cas9-based approach we engineer primary CD4+ T cells in which the LAT protein was tagged with an affinity Twin-Strep-tag (OST) with the purpose of determining by quantitative mass spectrometry the composition and dynamics of the signalosome assembling around LAT prior to and following T cell activation. Affinity purification of the OST tagged protein was performed using Streptactin beads, from T cells left non-stimulated, or stimulated for 30s or 120s with anti-CD3 and anti-CD4 antibodies. Each AP-MS purification is associated with a corresponding control (purification from non-edited WT CD4+ T cells, cultured and stimulated in the same conditions). The number of replicate biological experiments was n=3 for all conditions (time-points), and each sample was analyzed once by single-run nano LC-MS, resulting in 18 raw files.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:12:57.405.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne Gonzalez de Peredo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-14 02:34:21	ID requested
1	2020-09-29 03:34:38	announced
⏵ 2	2024-10-22 05:12:57	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: AP-MS,TCR, CRISPR/CAS9, interactome, primary CD+ T cell, OST tag

Odile Burlet-Schiltz
contact affiliation	Institute of Pharmacology and Structural Biology, CNRS, Toulouse
contact email	odile.schiltz@ipbs.fr
lab head
Anne Gonzalez de Peredo
contact affiliation	CNRS/IPBS
contact email	gonzalez@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD018526

PRIDE project URI

• PRIDE
  1. PXD018526
     1. Label: PRIDE project
     2. Name: Analysis of the interactome of LAT in CRISPR/CAS9 modified mouse CD+ T cells