PXD018517-1

PXD018517 is an original dataset announced via ProteomeXchange.

Title	The RNF185/Membralin ubiquitin ligase complex is a new ERAD branch involved in the degradation of a distinct subset of integral membrane proteins.
Description	The endoplasmic reticulum-associated degradation (ERAD) pathway is responsible for ubiquitin-mediated quality control of secretory and ER-resident proteins. In the present study, two short-lived ER integral membrane model proteins were used to screen a genome-wide CRISPR-Cas9 library, resulting in the identification of a new ERAD branch consisting of the RNF185/Membralin ubiquitin ligase complex. Biochemical and affinity pull-down studies followed by mass spectrometry revealed that the ubiquitin-like domain containing proteins TMUB-1 and -2 are also part of the core complex being specifically enriched by RNF185/Membralin but not by other known ERAD factors like HRD1. Genetic studies showed that the RNF185/Membralin complex control the degradation of endogenous integral membrane proteins, including TMUB2 itself and CYP51A1 from which the initial recombinant model protein was derived.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:39:21.691.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018517
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pedro Carvalho
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-14 01:53:32	ID requested
⏵ 1	2024-10-07 10:39:22	announced

10.1016/j.molcel.2020.07.009;

10.6019/PXD018517;

submitter keyword: Endoplasmic reticulum-associated degradation (ERAD)

E3 ligase RNF185

Membralin

TMEM259

TMUB1

TMUB2

ubiquitin

Pedro Carvalho
contact affiliation	Sir William Dunn School of Pathology University of Oxford United Kingdom
contact email	pedro.carvalho@path.ox.ac.uk
lab head
Pedro Carvalho
contact affiliation	University of Oxford
contact email	pedro.carvalho@path.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018517
     1. Label: PRIDE project
     2. Name: The RNF185/Membralin ubiquitin ligase complex is a new ERAD branch involved in the degradation of a distinct subset of integral membrane proteins.