PXD018446-2

PXD018446 is an original dataset announced via ProteomeXchange.

Title	Isolation and Characterization of small EVs from CHO-K1 cell cultures
Description	Chinese hamster ovary (CHO) cells are widely used host cells for recombinant protein production and currently the most commonly utilized mammalian organism in large scale biopharmaceutical production. Since the discovery of exosomes as a new type of small extracellular vesicles (EVs) by Johnstone et al., interest in EV research greatly increased in recent years. However, they yet pose a blank space in CHO research. Exosomes are 30 – 150 nm small vesicles, that derive from the endosomal network and can therefore be distinguished from plasma membrane-shed microvesicles (100 – 1000 nm in diameter) and apoptotic vesicles (50 – 5000 nm), which are secluded over the course of programmed cell death. It turned out exosomes are not only vehicles of cellular waste disposal, as was initially assumed, but also a conserved mechanism of cellular communication. This work aims to outline possible separation techniques followed by a differential proteomic and transcriptomic characterization of CHO EVs over the course of a bioreactor batch cultivation. Therefore, a protocol yielding sEVs with a strong exosomal marker enrichment is compared with HCP, lEVs and whole cell lysate (WCL) from the same batch process. This may allow for further studies in this field to have reference data for evaluation of exosome isolation techniques, separation purity and CHO EV composition in general.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:27:59.878.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louise Schelletter
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-09 04:20:05	ID requested
1	2021-09-09 00:31:44	announced
⏵ 2	2024-10-22 05:28:02	announced	2024-10-22: Updated project metadata.

10.1007/s00253-021-11309-8;

Keysberg C, Hertel O, Schelletter L, Busche T, Sochart C, Kalinowski J, Hoffrogge R, Otte K, Noll T, Exploring the molecular content of CHO exosomes during bioprocessing. Appl Microbiol Biotechnol, 105(9):3673-3689(2021) [pubmed]

submitter keyword: proteomics, CHO, cell culture,Extracellular vesicles, Exosomes

Thomas Noll
contact affiliation	Cell Culture Technology, Technical Faculty, Bielefeld University, Germany
contact email	thomas.noll@uni-bielefeld.de
lab head
Louise Schelletter
contact affiliation	Bielefeld University
contact email	l.brachtvogel@uni-bielefeld.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018446

PRIDE project URI

• PRIDE
  1. PXD018446
     1. Label: PRIDE project
     2. Name: Isolation and Characterization of small EVs from CHO-K1 cell cultures