PXD018423-3

PXD018423 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Binding proteins for the GA-modified proteins in mouse serum.
Description	We determined if the glycolaldehyde (GA)-modified proteins could show an affinity with serum proteins. The mouse serum was incubated with the ligand-coupled beads for 1 h at room temperature. The ligands used were BSA and GA-treated BSA (GA-BSA). The proteins bound to the beads were eluted and separated by SDS-PAGE. Seven unique bands detected in the pull-down with the GA-BSA ligands were processed for tryptic digestion and LC-MS/MS analysis.
HostingRepository	jPOST
AnnounceDate	2020-04-08
AnnouncementXML	Submission_2021-02-04_17:35:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lumi Negishi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-07 23:16:57	ID requested
1	2020-04-07 23:17:01	announced
2	2020-05-23 18:13:08	announced	2020-05-24: Updated PubMed.
⏵ 3	2021-02-04 17:35:19	announced	2021-02-04: Updated FTP location.
4	2022-09-18 03:46:20	announced	2022-09-18: Updated FTP location.

Publication List

Chikazawa M, Yoshitake J, Lim SY, Iwata S, Negishi L, Shibata T, Uchida K, -pyrrolation. J Biol Chem, 295(22):7697-7709(2020) [pubmed]

Chikazawa M, Yoshitake J, Lim SY, Iwata S, Negishi L, Shibata T, Uchida K, -pyrrolation. J Biol Chem, 295(22):7697-7709(2020) [pubmed]

Keyword List

submitter keyword: Glycolaldehyde, Mouse, Serum, Protein modification

submitter keyword: Glycolaldehyde, Mouse, Serum, Protein modification

Contact List

Koji Uchida
lab head
Lumi Negishi
contact affiliation	the University of Tokyo
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000787

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000787