PXD018383 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proximal Biotinylation Based Combinatory Approach for Isolating Plasma Membrane Proteins |
Description | Comprehensive profiling of the cell surface proteome has been challenging due to the lack of tools for an effective and reproducible way to isolate plasma membrane proteins from mammalian cells. Here, we employ a proximity-dependent biotinylation approach to label and isolate plasma membrane proteins without an extra in vitro labeling step that we called plasma membrane-BioID. The lipid modified BirA* enzyme (MyrPalm BirA*) was targeted to the plasma membrane where it effectively biotinylates plasma membrane proteins. Biotinylated proteins were then affinity purified and analyzed by mass spectrometry. Our analysis demonstrates that combining conventional sucrose density gradient centrifugation and plasma membrane-BioID is ideal to overcome the inherent limitations of identification of integral membrane proteins and yields highly pure plasma components for downstream proteomic analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-28 |
AnnouncementXML | Submission_2020-08-27_23:16:43.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Altuğ Kamacıoğlu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-06 01:21:12 | ID requested | |
⏵ 1 | 2020-08-27 23:16:44 | announced | |
Publication List
Akdag M, Yunt ZS, Kamacioglu A, Qureshi MH, Akarlar BA, Ozlu N, Proximal Biotinylation-Based Combinatory Approach for Isolating Integral Plasma Membrane Proteins. J Proteome Res, 19(8):3583-3592(2020) [pubmed] |
Keyword List
submitter keyword: cell surface, biotinylation, plasma membrane, sucrose density fractionation |
Contact List
Nurhan Ozlu |
contact affiliation | Department of Molecular Biology and Genetics, Koc University, Istanbul, Turkey |
contact email | nozlu@ku.edu.tr |
lab head | |
Altuğ Kamacıoğlu |
contact affiliation | Koç University |
contact email | akamacioglu18@ku.edu.tr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD018383 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018383
- Label: PRIDE project
- Name: Proximal Biotinylation Based Combinatory Approach for Isolating Plasma Membrane Proteins