PXD018382-1

PXD018382 is an original dataset announced via ProteomeXchange.

Title	Mycobacteria tolerate carbon monoxide by remodelling their respiratory chain
Description	Carbon monoxide (CO) is a gas infamous for its acute toxicity. The toxicity of CO predominately stems from its tendency to form carbonyl complexes with transition metals, thus inhibiting the heme-prosthetic groups of proteins, including the terminal oxidases of the respiratory chain. While CO has been proposed as an antibacterial agent, the evidence supporting its toxicity towards bacteria is equivocal, and its cellular targets remain poorly defined. In this work, we investigate the physiological response of Mycobacterium smegmatis to CO. We show that M. smegmatis is highly resistant to the toxic effects of CO, exhibiting normal growth parameters when cultured in its presence. We profiled the proteome of M. smegmatis during growth in CO, identifying strong induction of cytochrome bd oxidase and members of the dos regulon, but relatively few other changes. We show that the activity of cytochrome bd oxidase is resistant to CO, whereas cytochrome bcc-aa3 oxidase is strongly inhibited by this gas. Consistent with these findings, growth analysis shows that M. smegmatis lacking cytochrome bd oxidase displays a significant growth defect in the presence of CO, while induction of the dos regulon appears to be unimportant for adaption to CO. Altogether, our findings suggest that M. smegmatis has considerable resistance to CO and benefits from respiratory flexibility to withstand its inhibitory effects.
HostingRepository	PRIDE
AnnounceDate	2021-04-30
AnnouncementXML	Submission_2021-04-30_00:33:10.124.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cheng Huang
SpeciesList	scientific name: Mycobacterium sp. GN-10803; NCBI TaxID: 632138;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-06 01:21:06	ID requested
⏵ 1	2021-04-30 00:33:10	announced

Dataset with its publication pending

submitter keyword: Carbon monoxide, respiratory chain, Mycobacteria

Chris Greening
contact affiliation	Department of Microbiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia
contact email	chris.greening@monash.edu
lab head
Cheng Huang
contact affiliation	Monash University
contact email	cheng.huang@monash.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018382
     1. Label: PRIDE project
     2. Name: Mycobacteria tolerate carbon monoxide by remodelling their respiratory chain