PXD018373 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Conserved protein Pir2ARS2 mediates gene repression through cryptic introns in lncRNAs |
Description | Long non-coding RNAs (lncRNAs) are components of epigenetic control mechanisms that ensure appropriate and timely gene expression. The functions of lncRNAs are often mediated through associated gene regulatory activities, but how lncRNAs are distinguished from other RNAs and recruit effector complexes is unclear. Here we utilize the fission yeast Schizosaccharomyces pombe to investigate how lncRNAs engage silencing activities to regulate gene expression in cis. We find that invasion of lncRNA transcription into the downstream gene body incorporates a cryptic intron required for repression of that gene. Our analyses show that lncRNAs containing cryptic introns are targeted by the conserved Pir2ARS2 protein in association with splicing factors, which recruit RNA processing and chromatin modifying activities involved in gene silencing. Pir2 and splicing machinery are broadly required for gene repression. Our finding that human ARS2 also interacts with splicing factors suggests a conserved mechanism mediates gene repression through cryptic introns within lncRNAs. |
HostingRepository | PRIDE |
AnnounceDate | 2020-04-07 |
AnnouncementXML | Submission_2020-04-06_22:46:16.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD018373 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Lisa Jenkins |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Acetyl; Carbamidomethyl |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-04 10:18:27 | ID requested | |
⏵ 1 | 2020-04-06 22:46:17 | announced | |
2 | 2020-05-21 23:29:36 | announced | 2020-05-22: Updated publication reference for PubMed record(s): 32415063. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: RIME, lncRNA, ARS2 |
Contact List
Lisa M. Jenkins |
contact affiliation | Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH |
contact email | lisa.jenkins@nih.gov |
lab head | |
Lisa Jenkins |
contact affiliation | National Cancer Institute, NIH |
contact email | lisa.jenkins@nih.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018373
- Label: PRIDE project
- Name: Conserved protein Pir2ARS2 mediates gene repression through cryptic introns in lncRNAs