PXD018295-1

PXD018295 is an original dataset announced via ProteomeXchange.

Title	Improvements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications
Description	Trypanosome histone N-terminal sequences are very divergent from the other eukaryotes, although they are still decorated by post-translational modifications (PTMs). Here, we used a highly robust workflow to analyze histone PTMs in the parasite Trypanosoma cruzi using mass spectrometry-based data-independent acquisition (DIA). We adapted the workflow for the analysis of the parasite’s histone sequences by modifying the software EpiProfile 2.0, improving peptide and PTM quantification accuracy. This workflow could now be applied to the study of 141 T. cruzi modified histone peptides, which we used to investigate the dynamics of histone PTMs along the metacyclogenesis and the life cycle of T. cruzi.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:33:10.019.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Cunha
SpeciesList	scientific name: Trypanosoma cruzi cruzi; NCBI TaxID: 85057;
ModificationList	monomethylated residue; phosphorylated residue; acetylated residue; dimethylated residue; N6,N6,N6-trimethyl-L-lysine
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-30 23:40:07	ID requested
⏵ 1	2024-10-07 13:33:10	announced

Dataset with its publication pending

submitter keyword: PTM, Trypanosoma cruzi,Histone

Julia da Cunha
contact affiliation	Cell Cycle Laboratory, Butantan Institute, Brazil
contact email	julia.cunha@butantan.gov.br
lab head
Julia Cunha
contact affiliation	LCC
contact email	julia.cunha@butantan.gov.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD018295

PRIDE project URI

• PRIDE
  1. PXD018295
     1. Label: PRIDE project
     2. Name: Improvements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications