PXD018230-1

PXD018230 is an original dataset announced via ProteomeXchange.

Title	Functional states in tumor-initiating cell differentiation in human colorectal cancer
Description	Intra-tumor heterogeneity of tumor-initiating cell (TIC) activity drives colorectal cancer (CRC) progression and therapy resistance. Here, we used single-cell mRNA-sequencing (scRNA-seq) of patient-derived CRC models to decipher distinct cell subpopulations based on their transcriptional profiles. Cell type-specific expression modules of stem-like, transit amplifying-like, and differentiated CRC cells resemble differentiation states of normal intestinal epithelial cells. Strikingly, identified subpopulations differ in proliferative activity and metabolic state. In summary, we here show at single-cell resolution that transcriptional heterogeneity identifies functional states during TIC differentiation. Targeting transcriptional states associated to cancer cell differentiation might unravel vulnerabilities in human CRC.
HostingRepository	PRIDE
AnnounceDate	2021-05-07
AnnouncementXML	Submission_2021-05-07_02:54:51.664.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Torsten Mueller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-27 02:53:17	ID requested
⏵ 1	2021-05-07 02:54:52	announced
2	2021-05-09 22:18:49	announced	2021-05-10: Updated project metadata.
3	2024-10-22 05:21:59	announced	2024-10-22: Updated project metadata.

10.3390/CANCERS13051097;

submitter keyword: colorectal cancer, tumor-initiating cells, tumor heterogeneity, patient-derived cancer models, single-cell RNA-sequencing, tumor metabolism, transcriptional programs, tumor cell differentiation

Claudia R. Ball
contact affiliation	NCT Dresden
contact email	claudia.ball@nct-dresden.de
lab head
Torsten Mueller
contact affiliation	DKFZ
contact email	torsten.mueller84@googlemail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD018230

PRIDE project URI

• PRIDE
  1. PXD018230
     1. Label: PRIDE project
     2. Name: Functional states in tumor-initiating cell differentiation in human colorectal cancer